Mendelsohn F A, Allen A M, Clevers J, Denton D A, Tarjan E, McKinley M J
Department of Medicine, Austin Hospital, University of Melbourne, Heidelberg, Australia.
J Comp Neurol. 1988 Apr 15;270(3):372-84. doi: 10.1002/cne.902700306.
Binding of 125I-[Sar1,Ile8] angiotensin II (AII) to sections of brains from both wild and laboratory rabbits was determined by in vitro autoradiography. In the forebrain, specific high density binding was observed in the olfactory bulb, organum vasculosum of the lamina terminalis (OVLT), subfornical organ, median eminence, lateral septum, median preoptic nucleus and hypothalamic paraventricular, supraoptic and arcuate nuclei. In the midbrain, binding of the radioligand was observed in the interpeduncular and parabrachial nuclei, in the locus coeruleus, and ventrolateral pons. In the hind brain, there was dense binding of 125I-[Sar1,Ile8] AII to the nucleus of the solitary tract (NTS) and to both rostral and caudal parts of the reticular formation of the ventrolateral medulla oblongata. Weaker specific binding of the radioligand to the molecular layer of the cerebellum, to the nucleus of the spinal trigeminal tract, dorsal motor nucleus of the vagus, area postema, and to a band of tissue connecting the NTS to the ventrolateral medulla was also observed. Binding of the ligand to circumventricular organs such as the OVLT, subfornical organ, and median eminence suggests that these are sites in the brain of the rabbit at which blood-borne AII may exert influences on the central regulation of fluid balance and pituitary hormone secretion, although AII of neuronal origin could also act at these sites. Binding of the radioligand in several other brain regions suggests that angiotensin II of cerebral origin may be involved in a number of different aspects of brain function in the rabbit. The finding of dense binding in the NTS and ventrolateral medulla, which are involved in autonomic activity and are also sites of catecholamine-containing neurons, raises the possibility of angiotensin interaction with these neurons and involvement in autonomic function.
通过体外放射自显影法测定了125I-[Sar1，Ile8]血管紧张素II（AII）与野生兔和实验兔脑切片的结合情况。在前脑，在嗅球、终板血管器（OVLT）、穹窿下器官、正中隆起、外侧隔、视前正中核以及下丘脑室旁核、视上核和弓状核中观察到特异性高密度结合。在中脑，放射性配体在脚间核、臂旁核、蓝斑和脑桥腹外侧被观察到有结合。在后脑，125I-[Sar1，Ile8] AII与孤束核（NTS）以及延髓腹外侧网状结构的头端和尾端有密集结合。还观察到放射性配体与小脑分子层、三叉神经脊束核、迷走神经背运动核、最后区以及连接NTS与延髓腹外侧的一条组织带的特异性结合较弱。配体与OVLT、穹窿下器官和正中隆起等室周器官的结合表明，这些是兔脑中血源性AII可能对液体平衡的中枢调节和垂体激素分泌产生影响的部位，尽管神经元来源的AII也可能在这些部位起作用。放射性配体在其他几个脑区的结合表明，脑源性血管紧张素II可能参与兔脑功能的许多不同方面。在参与自主活动且也是含儿茶酚胺神经元所在部位的NTS和延髓腹外侧发现密集结合，这增加了血管紧张素与这些神经元相互作用并参与自主功能调节的可能性。
