Allen A M, Paxinos G, McKinley M J, Chai S Y, Mendelsohn F A
University of Melbourne Department of Medicine, Austin Hospital, Heidelberg, Victoria, Australia.
J Comp Neurol. 1991 Oct 8;312(2):291-8. doi: 10.1002/cne.903120211.
Angiotensin II (Ang II) binding sites were localized in the thalamus, basal ganglia, midbrain, and pons of the human central nervous system by in vitro autoradiography, employing 125I-[Sar1, Ile8]angiotensin II as the radioligand. High-density binding occurs in the substantia nigra pars compacta, the interpeduncular nucleus and two of the raphe nuclei, the raphe magnus, and median raphe nucleus. Moderate densities occur in the caudate nucleus, putamen, bed nucleus of the stria terminalis, rostral linear nucleus, caudal linear nucleus, dorsal and paramedian raphe nuclei, locus coeruleus, and region of the subcoeruleus, oral dorsal paramedian nucleus, and A5/periolivary region. Low levels occur in the region between the subthalamic nucleus and the zona incerta, the mediodorsal thalamic nucleus, the central gray, the lateral and medial parabrachial nuclei, and the molecular layer of the cerebellum. The high density of Ang II receptor binding in the substantia nigra occurs over pigmented, presumably dopaminergic, neurons. The binding in this site, and in the striatum, is not observed in any of the other species we have studied. It displays similar pharmacological characteristics to the Ang II receptor binding site in other regions of the human brain. Overall we demonstrate a discrete pattern of Ang II receptor binding sites in the human brain, which shows a high correlation with the distribution observed in other mammalian species.
采用¹²⁵I - [Sar¹, Ile⁸]血管紧张素II作为放射性配体，通过体外放射自显影法，将血管紧张素II（Ang II）结合位点定位在人类中枢神经系统的丘脑、基底神经节、中脑和脑桥。高密度结合出现在黑质致密部、脚间核以及两个中缝核，即中缝大核和中缝正中核。中等密度出现在尾状核、壳核、终纹床核、嘴侧线性核、尾侧线性核、背侧和旁正中中缝核、蓝斑以及蓝斑下区域、嘴侧背侧旁正中核和A5/橄榄周区域。低水平出现在丘脑底核和未定带之间的区域、丘脑背内侧核、中央灰质、外侧和内侧臂旁核以及小脑分子层。黑质中高密度的Ang II受体结合出现在色素沉着的、可能是多巴胺能的神经元上。在我们研究的任何其他物种中，均未观察到该位点以及纹状体中的结合情况。它在人类大脑其他区域与Ang II受体结合位点表现出相似的药理学特征。总体而言，我们证明了人类大脑中Ang II受体结合位点的离散模式，该模式与在其他哺乳动物物种中观察到的分布高度相关。
