Department of Chemistry, Campus Delivery 1872, Colorado State Unviersity, Fort Collins, CO, 80523-1827, USA.
Department of Physics, University of Nebraska - Omaha, Omaha, NE, 68182, USA.
Sci Rep. 2021 Mar 16;11(1):6070. doi: 10.1038/s41598-021-85611-w.
The majority of biological processes are regulated by enzymes, precise control over specific enzymes could create the potential for controlling cellular processes remotely. We show that the thermophilic enzyme thermolysin can be remotely activated in 17.76 MHz radiofrequency (RF) fields when covalently attached to 6.1 nm gold coated magnetite nanoparticles. Without raising the bulk solution temperature, we observe enzyme activity as if the solution was 16 ± 2 °C warmer in RF fields-an increase in enzymatic rate of 129 ± 8%. Kinetics studies show that the activity increase of the enzyme is consistent with the induced fit of a hot enzyme with cold substrate.
大多数生物过程都受到酶的调节，对特定酶的精确控制可能为远程控制细胞过程创造潜力。我们表明，当共价附着在 6.1nm 金涂覆磁铁矿纳米颗粒上时，嗜热酶 thermolysin 可以在 17.76MHz 射频 (RF) 场中被远程激活。在不提高溶液的体相温度的情况下，我们观察到酶的活性，就好像溶液在 RF 场中升温了 16±2°C-酶反应速率提高了 129±8%。动力学研究表明，酶活性的增加与冷底物的热酶的诱导契合一致。
