Andreeva Yulia I, Drozdov Andrey S, Avnir David, Vinogradov Vladimir V
Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, St. Petersburg 197101, Russian Federation.
Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
ACS Biomater Sci Eng. 2018 Dec 10;4(12):3962-3967. doi: 10.1021/acsbiomaterials.8b00838. Epub 2018 Nov 6.
The control over enzymatic activity by physical stimuli is of interest to many applications in medicine, biotechnology, synthetic biology, and nanobionics. Although the main focus has been on optically responsive systems, alternative strategies to modulate the enzymatic activity of hybrid systems are needed. Here we describe a radiofrequency (RF) field controlled catalytic activity of an enzymatic sol-gel composite. Specifically, the activity of bovine carbonic anhydrase entrapped in sol-gel-derived magnetite (enzyme@ferria) composite was accelerated by a factor of 460% compared to its initial value, by applying the RF field of 937 A/m, with fast response time. This acceleration is reversible and its magnitude controllable. An acceleration mechanism, based on RF-induced heating of the magnetite by the Néel relaxation effect, is proposed and proven. The entrapment within a sol-gel matrix solves the problem of enhancing activity by heating without denaturing the enzyme. RF-controlled enzymatic composites can be potentially applied as biological RF sensors or to control biochemical reactions within living organisms.
通过物理刺激控制酶活性在医学、生物技术、合成生物学和纳米生物等诸多应用领域备受关注。尽管主要关注点一直是光响应系统，但仍需要其他策略来调节混合系统的酶活性。在此，我们描述了一种酶促溶胶 - 凝胶复合材料的射频（RF）场控制催化活性。具体而言，通过施加937 A/m的RF场，包埋在溶胶 - 凝胶衍生磁铁矿（酶@铁氧体）复合材料中的牛碳酸酐酶活性与其初始值相比提高了460%，且响应时间快。这种加速是可逆的，其幅度可控。基于奈尔弛豫效应通过RF诱导磁铁矿加热的加速机制被提出并得到证实。包埋在溶胶 - 凝胶基质中解决了通过加热增强活性而不使酶变性的问题。RF控制的酶复合材料有潜力用作生物RF传感器或用于控制活体内的生化反应。
