College of Chemistry and Materials Science, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, 1 Xue Fu Avenue, Xi'an, 710127, Shaanxi, China.
Nanoscale. 2019 Oct 10;11(39):18081-18089. doi: 10.1039/c9nr04289j.
Remote modulation of nanoscale biochemical processes in a living system using magnetic stimulation is appealing but is restricted by the lack of a highly efficient nanomediator which can deliver timely and effective response to biological molecules under an external magnetic field. Herein, we report the development of a novel nanocatalyst based on a ferrimagnetic vortex-domain nanoring (FVIO)-enzyme hybrid that enables real-time modulation of enzymatic catalysis under an alternating magnetic field (AMF). The role of the FVIO is to provide localized heating immediately upon exposure to an AMF, which efficiently and selectively promotes the activity of conjugated enzymes on the surface. The reaction rate of the as-fabricated FVIO-β-Gal hybrid was shown to be boosted up to 180% of its initial value by localized heat generated under an AMF of 550 Oe in less than 2 s and without heating up the bulk solution. Moreover, the degree of activity acceleration was shown to be tunable by increasing the strength of the AMF. The concept of remote magnetic stimulation of enzymatic reactions has been further applied to other enzymes (e.g. FVIO-KPC and FVIO-GOx), demonstrating the general applicability of this strategy. Since almost all metabolic processes in cells rely on enzymatic catalysis to sustain life, the FVIO-enzyme system developed in this work provides a valuable nanoplatform for spatiotemporally manipulating biochemical reactions, which might pave the way for future remote manipulation of living organisms.
利用磁场刺激远程调节活系统中的纳米级生化过程很有吸引力，但受到缺乏高效纳米介体的限制，这种纳米介体能在外磁场下及时有效地响应生物分子。在此，我们报告了一种基于亚铁磁涡旋畴纳米环（FVIO）-酶杂化体的新型纳米催化剂的开发，该催化剂能够在交变磁场（AMF）下实时调节酶催化。FVIO 的作用是在暴露于 AMF 时立即提供局部加热，从而有效地和选择性地促进表面上共轭酶的活性。所制备的 FVIO-β-Gal 杂化体的反应速率在不到 2 秒的时间内通过在 550 Oe 的 AMF 下产生的局部热量提高到初始值的 180%，而不会使本体溶液升温。此外，通过增加 AMF 的强度，可以调节活性加速的程度。远程磁刺激酶反应的概念已进一步应用于其他酶（例如 FVIO-KPC 和 FVIO-GOx），证明了该策略的通用性。由于细胞中几乎所有的代谢过程都依赖于酶催化来维持生命，因此本工作中开发的 FVIO-酶系统为时空操控生化反应提供了一个有价值的纳米平台，这可能为未来对生物体的远程操控铺平道路。
