Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
Department of Ophthalmology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.
Eur J Immunol. 2021 Jul;51(7):1850-1853. doi: 10.1002/eji.202049083. Epub 2021 Mar 29.
Generation of TCR-like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip-based Ab-secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single-cell level to obtain TCR-like antibodies; blocking procedure enables us to avoid the detection of non-TCR-like antibodies.
使用传统方法生成 TCR 样单克隆抗体非常费力且效率低下。我们提出了 ISAAC（基于芯片的 Ab 分泌细胞 [ASC] 筛选方法）的改进方法，允许在单细胞水平上全面分析 ASC 以获得 TCR 样抗体；阻断程序使我们能够避免检测非 TCR 样抗体。
