Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.
Technical Innovation Center for Utilization of Marine Biological Resources, Ministry of Natural Resources, Xiamen, China.
J Basic Microbiol. 2021 May;61(5):406-418. doi: 10.1002/jobm.202000728. Epub 2021 Mar 17.
Seaweed oligosaccharides possess great bioactivities. However, different microbial strains are required to degrade multiple polysaccharides due to their limited biodegradability, thereby increasing the cost and complexity of production. Shewanella sp. WPAGA9 was isolated from deep-sea sediments in this study. According to the genomic and biochemical analyses, the extracellular fermentation broth of WPAGA9 had versatile degradation abilities for three typical seaweed polysaccharides including agar, carrageenan, and alginate. The maximum enzyme activities of the extracellular fermentation broth of WPAGA9 were 71.63, 76.4, and 735.13 U/ml for the degradation of agar, alginate, and carrageenan, respectively. Moreover, multiple seaweed oligosaccharides can be produced by the extracellular fermentation broth of WPAGA9 under similar optimum conditions. Therefore, WPAGA9 can simultaneously degrade three types of seaweed polysaccharides under similar conditions, thereby greatly reducing the production cost of seaweed oligosaccharides. This finding indicates that Shewanella sp. WPAGA9 is an ideal biochemical tool for producing multiple active seaweed oligosaccharides at low costs and is also an important participant in the carbon cycle process of the deep-sea environment.
海藻寡糖具有巨大的生物活性。然而，由于其生物降解性有限，需要不同的微生物菌株来降解多种多糖，从而增加了生产成本和生产的复杂性。本研究从深海沉积物中分离到一株希瓦氏菌 WPAGA9。根据基因组和生化分析，WPAGA9 的胞外发酵液对琼脂、卡拉胶和褐藻胶这三种典型的海藻多糖具有多种降解能力。WPAGA9 胞外发酵液对琼脂、褐藻胶和卡拉胶的最大酶活分别为 71.63、76.4 和 735.13 U/ml。此外，WPAGA9 的胞外发酵液在相似的最佳条件下可以产生多种海藻寡糖。因此，WPAGA9 可以在相似条件下同时降解三种类型的海藻多糖，从而大大降低了海藻寡糖的生产成本。这一发现表明，希瓦氏菌 WPAGA9 是一种理想的低成本生产多种活性海藻寡糖的生化工具，也是深海环境碳循环过程中的重要参与者。
