Wang Fengping, Wang Peng, Chen Mingxia, Xiao Xiang
Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen, PR China.
Extremophiles. 2004 Apr;8(2):165-8. doi: 10.1007/s00792-003-0365-0. Epub 2003 Dec 13.
Tests to detect the presence of piezophilic Shewanella strains in the deep-sea sediments of the west, mid- and east Pacific at different depths were done by amplification of previously identified pressure-regulated operons (ORF1,2 and ORF3). The operon fragments were detected in all the deep-sea sediment samples, indicating the broad presence of piezophilic deep-sea Shewanella species or related species in the deep-sea sediments across the Pacific. Extremophiles were isolated from the deep-sea sediment of the west Pacific under atmospheric pressure. Two psychrophilic/psychrotrophic strains, WP2 and WP3, were assigned to the Shewanella genus as determined by their 16S rDNA sequences. WP2 and WP3 were both capable of amplifying pressure-regulated operons; the sequences of the pressure-regulated operons of WP2 and WP3 share high identity between each other, but have more differences from those of S. benthica and S. violacea. The major fatty acids of WP2 and WP3 are 3OH-i-13:0, 14:0, i-15:0, 16:0, 16:1, 18:1, and 20:5. Combined phenotypic analysis, 16S rDNA sequences, and DNA-DNA hybridization results suggest that WP2 and WP3 are two new deep-sea Shewanella species.
通过扩增先前鉴定的压力调节操纵子（ORF1、2和ORF3），对西太平洋、中太平洋和东太平洋不同深度深海沉积物中嗜压希瓦氏菌菌株的存在情况进行了检测。在所有深海沉积物样本中均检测到了操纵子片段，这表明嗜压深海希瓦氏菌物种或相关物种广泛存在于太平洋的深海沉积物中。在常压下从西太平洋深海沉积物中分离出了极端微生物。通过16S rDNA序列测定，将两株嗜冷/耐冷菌株WP2和WP3归为希瓦氏菌属。WP2和WP3均能够扩增压力调节操纵子；WP2和WP3的压力调节操纵子序列彼此之间具有高度同一性，但与底栖希瓦氏菌和紫色希瓦氏菌的操纵子序列存在更多差异。WP2和WP3的主要脂肪酸为3OH-i-13:0、14:0、i-15:0、16:0、16:1、18:1和20:5。综合表型分析、16S rDNA序列和DNA-DNA杂交结果表明，WP2和WP3是两个新的深海希瓦氏菌物种。
