State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Center for Disease Control and Prevention, Guangdong Provincial Institute of Public Health, Guangzhou, China.
PLoS Pathog. 2021 Mar 17;17(3):e1009396. doi: 10.1371/journal.ppat.1009396. eCollection 2021 Mar.
Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions' associations with the extracellular transmission of OmRV.
蚊虫病毒会引发不可预测的疾病爆发。最近，从蚊子中分离出的几种未分类病毒，包括Omono 河病毒（OmRV），被鉴定为类 totivirus 病毒，具有类似于 Totiviridae 家族的特征。该家族中大多数报道的成员感染真菌或原生动物，并且缺乏细胞外生活史阶段。在这里，我们鉴定了一种新的 OmRV 株，并使用单颗粒冷冻电镜确定了该病毒的高分辨率结构。该结构在衣壳的五重顶点处具有意想不到的突起。突起的解离可能导致主要衣壳发生几种构象变化。所有这些结构以及一些生物学结果表明，突起与 OmRV 的细胞外传播有关。
