Chen Yi-Sin, Lai Charles Pin-Kuang, Chen Chihchen, Lee Gwo-Bin
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan and Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan and Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, Taiwan.
Lab Chip. 2021 Apr 20;21(8):1475-1483. doi: 10.1039/d1lc00093d.
Cell-released, membrane-encapsulated extracellular vesicles (EVs) serve as a means of intercellular communication by delivering bioactive cargos including proteins, nucleic acids and lipids. EVs have been widely used for a variety of biomedical applications such as biomarkers for disease diagnosis and drug delivery vehicles for therapy. Herein, this study reports a novel method for label-free, contact-free isolation and recovery of EVs via optically-induced dielectrophoresis (ODEP) on a pneumatically-driven microfluidic platform with minimal human intervention. At an optimal driving frequency of 20 kHz and a voltage of 20 Vpp, an ODEP force from a 75 μm moving light beam was characterized to be 23.5-97.7 fN in 0.2 M sucrose solution. Furthermore, rapid enrichment of EVs with a small volume of only 27 pL in 32 s achieved an increase of 272-fold by dynamically shrinking circular light patterns. Moreover, EVs could be automatically isolated and recovered within 25 min, while achieving a releasing efficiency of 99.8% and a recovery rate of 52.2% by using an integrated microfluidics-based optically-induced EV isolation (OIEV) platform. Given the capacity of label-free, contact-free EV isolation, and automatic, easy-releasing EV recovery, this integrated OIEV platform provides a unique approach for EV-based disease diagnosis and drug delivery applications.
细胞释放的、被膜包裹的细胞外囊泡（EVs）通过传递包括蛋白质、核酸和脂质在内的生物活性物质，作为细胞间通讯的一种方式。EVs已被广泛应用于各种生物医学领域，如疾病诊断的生物标志物和治疗的药物递送载体。在此，本研究报告了一种在气动微流控平台上通过光诱导介电泳（ODEP）实现无标记、非接触式分离和回收EVs的新方法，且人工干预极少。在20 kHz的最佳驱动频率和20 Vpp的电压下，在0.2 M蔗糖溶液中，来自75μm移动光束的ODEP力被表征为23.5 - 97.7 fN。此外，通过动态收缩圆形光图案，在32秒内仅用27 pL的小体积快速富集EVs，实现了272倍的增加。而且，使用基于微流控的集成光诱导EV分离（OIEV）平台，EVs可在25分钟内自动分离和回收，同时实现99.8%的释放效率和52.2%的回收率。鉴于其无标记、非接触式分离EVs以及自动、易于释放回收EVs的能力，这种集成的OIEV平台为基于EVs的疾病诊断和药物递送应用提供了一种独特的方法。
