Han Bo Hoon, Kim Sumi, Seo Geeyoon, Heo Youhee, Chung Seok, Kang Ji Yoon
Center for BioMicrosystems, Korea Institute of Science and Technology, Seoul, Republic of Korea.
School of Mechanical Engineering, Korea University, Seoul, Korea and KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea.
Lab Chip. 2020 Sep 29;20(19):3552-3559. doi: 10.1039/d0lc00345j.
As conventional bulky methods for extracellular vesicle (EV) separation are unsuitable for small volumes of samples, microfluidic devices are thought to offer a solution for the integrated and automatic processing of EV separation. This study demonstrates a simple microfluidic aqueous two-phase system (ATPS) for EV separation with high recovery efficiency to overcome the limitation of previous devices, which require complex external equipment or high cost manufacturing. With polyethylene glycol and dextran in the microfluidic channel, the isolation mechanism of the microfluidic ATPS was analyzed by comparison between two-phase and one-phase systems. Our device could facilitate continuous EV isolation with 83.4% recovery efficiency and remove 65.4% of the proteins from the EV-protein mixture. EVs were also successfully isolated from human plasma at high recovery efficiency.
由于传统的用于细胞外囊泡（EV）分离的庞大方法不适用于小体积样本，微流控设备被认为可为EV分离的集成化和自动化处理提供解决方案。本研究展示了一种用于EV分离的简单微流控水两相系统（ATPS），具有高回收效率，以克服先前设备的局限性，先前设备需要复杂的外部设备或高成本制造。通过在微流控通道中使用聚乙二醇和葡聚糖，通过两相和单相系统的比较分析了微流控ATPS的分离机制。我们的设备可以促进EV的连续分离，回收效率为83.4%，并从EV-蛋白质混合物中去除65.4%的蛋白质。还以高回收效率成功地从人血浆中分离出了EV。
