Acuvet Biotech, C/Bari 25 dpdo, Zaragoza, 50197, Spain.
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, Lublin, 20-612, Poland.
Vet Immunol Immunopathol. 2021 May;235:110221. doi: 10.1016/j.vetimm.2021.110221. Epub 2021 Mar 1.
Measurement of acute phase proteins (APPs) as biomarkers in canine medicine is in increasing demand. In the present study, the development and validation of two ELISA methods for the quantification of canine inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) and haptoglobin (Hp) are shown. The adequate imprecision and accuracy and wide analytical range make the developed methods appropriate to quantify ITIH4 and Hp in serum samples. The inter- and intra-assay CVs were lower than 10 %, and the assays maintained linearity under dilution and showed analytical equivalence with the method of radial immunodiffusion. The measurement of CRP, Hp and ITIH4 in sera from bitches affected by pyometra allowed us to determine that ITIH4 behaves as a moderate APP in dogs. The group of bitches affected by pyometra showed very high levels of CRP (147 ± 91 mg/L), corresponding to a strong inflammatory process, which resulted in a moderate increase in the concentrations of Hp (7 times) and ITIH4 (3 times) compared to the control group.
在犬医学中，作为生物标志物的急性相蛋白（APPs）的测量需求正在不断增加。本研究展示了两种用于定量检测犬型α-胰蛋白酶抑制剂重链 4（ITIH4）和触珠蛋白（Hp）的 ELISA 方法的开发和验证。所开发的方法具有足够的精密度和准确度以及较宽的分析范围，适用于定量检测血清样本中的 ITIH4 和 Hp。批内和批间 CV 均低于 10%，且该方法在稀释时保持线性，并与放射免疫扩散法具有分析等效性。测量患有子宫蓄脓的母犬的 CRP、Hp 和 ITIH4，使我们能够确定 ITIH4 在犬中是一种中等程度的 APP。患有子宫蓄脓的犬组的 CRP 水平非常高（147±91mg/L），对应于强烈的炎症过程，这导致 Hp（7 倍）和 ITIH4（3 倍）的浓度与对照组相比中度增加。
