He L F, Hou X X, Chen T, Zhang L, Wen S, Miao G Q, Xing M, Hao Q, Zhu X
Department of Central Laboratory, People's Hospital of Sanya, Sanya 572000, China.
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2021 Mar 6;55(3):379-385. doi: 10.3760/cma.j.cn112150-20200527-00787.
To understand the infectious status of Lyme disease among patients with arthritis symptoms in Hainan Province, and to provide a theoretical basis for prevention and control of Lyme disease. From 2013 to 2018, sampling surveys had been conducted in medical institutions in 8 cities in Hainan Province(Haikou, Sanya, Danzhou, Dongfang, Wenchang, Qionghai, Qiongzhong, Wuzhishan), 2 311 patients serum samples were collected with arthritis symptoms, and descriptive research were conducted base on the collected clinical data. The Indirect Fluorescent-Antibody Test (IFA) method was used for preliminary screening of Lyme disease antibody, the Western Blot (WB) method was used for IFA positive samples confirmation. Statistical analysis using test. 2 311 serum samples were tested by IFA, and 166 were positive with the positive rate of 7.18%. Further confirmed by WB method, 62 samples were positive, the positive rate of Lyme disease antibody was 2.68%(62/2 311). The positive rate of Lyme disease antibody among patients with arthritis in different regions of Hainan was statistically significant (²40.636,<0.001), and the positive rate in Qiongzhong city was the highest (8.81%, 14/159). Danzhou's positive rate was the second highest, 5.62%(5/89). Dongfang city had the lowest positive rate (0.51%, 2/394). The positive rates of Lyme disease serum antibody in men and women were 2.79% (33/1 182) and 2.57% (29/1 129), respectively; the positive rates of antibodies between each age groups were in the range of 1.74% to 3.64%. The antibody positive rate of Lyme disease showed no significant difference between gender and age (²0.110,=0.740 ;²1.938,=0.747). Patients with arthritis symptoms caused by Borrelia burgdorferi infection were found in 8 cities in Hainan province, but the Lyme disease antibody positive rate was different among cities, with Qiongzhong County being the highest.
了解海南省关节炎症状患者中莱姆病的感染状况，为莱姆病的防控提供理论依据。2013年至2018年，对海南省8个市（海口、三亚、儋州、东方、文昌、琼海、琼中、五指山）的医疗机构进行抽样调查，收集2311例有关节炎症状患者的血清样本，并基于所收集的临床资料进行描述性研究。采用间接荧光抗体试验（IFA）方法对莱姆病抗体进行初步筛查，采用免疫印迹法（WB）对IFA阳性样本进行确认。采用检验进行统计分析。2311份血清样本经IFA检测，166份呈阳性，阳性率为7.18%。经WB法进一步确认，62份样本呈阳性，莱姆病抗体阳性率为2.68%（62/2311）。海南省不同地区关节炎患者莱姆病抗体阳性率差异有统计学意义（² = 40.636，<0.001），琼中市阳性率最高（8.81%，14/159）。儋州阳性率次之，为5.62%（5/89）。东方市阳性率最低（0.51%，2/394）。男性和女性莱姆病血清抗体阳性率分别为2.79%（33/1182）和2.57%（29/1129）；各年龄组抗体阳性率在1.74%至3.64%之间。莱姆病抗体阳性率在性别和年龄之间差异无统计学意义（² = 0.110， = 0.740；² = 1.938， = 0.747）。在海南省8个市发现了由伯氏疏螺旋体感染引起关节炎症状的患者，但不同市之间莱姆病抗体阳性率不同，琼中县最高。
