Manuschai Jutharat, Sotozono Maki, Takenaka Shoji, Kornsombut Niraya, Takahashi Ryouhei, Saito Rui, Nagata Ryoko, Ida Takako, Noiri Yuichiro
Division of Cariology, Operative Dentistry and Endodontics, Faculty of Dentistry, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan.
Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.
Antibiotics (Basel). 2024 Aug 7;13(8):743. doi: 10.3390/antibiotics13080743.
Applying a saturated potassium iodide (KI) solution immediately after silver diamine fluoride (SDF) application may affect the inhibitory effects of SDF on biofilm formation. This study compared the efficacy of 38% SDF with and without KI on preventing mixed-species biofilm formation on human root dentin surfaces and assessed ion incorporation into root dentin. The biofilms, composed of , , and , were grown on specimen surfaces treated with either SDF or SDF + KI. After 24 h, the biofilms were evaluated using scanning electron microscopy, live/dead staining, adenosine triphosphate (ATP) assays, colony-forming unit (CFU) counts, and quantitative polymerase chain reaction. A Mann-Whitney test was used to compare the results between the groups. Ion incorporation was assessed using an electron probe microanalyzer. The relative ATP content in the SDF + KI group was significantly higher than that in the SDF group ( < 0.05). However, biofilm morphology and the logarithmic reduction in CFUs and bacterial DNA were comparable across the groups. The SDF + KI treatment resulted in less silver and fluoride ion incorporation than that yielded by SDF alone. The inhibitory effects of SDF and SDF + KI on mixed-species biofilm formation were almost equivalent, although KI application affected the ion incorporation.
在应用氟化亚锡（SDF）后立即应用饱和碘化钾（KI）溶液可能会影响SDF对生物膜形成的抑制作用。本研究比较了含KI和不含KI的38% SDF在预防人牙根牙本质表面混合菌种生物膜形成方面的效果，并评估了离子向牙根牙本质中的掺入情况。由[具体菌种1]、[具体菌种2]和[具体菌种3]组成的生物膜在经SDF或SDF + KI处理的标本表面生长。24小时后，使用扫描电子显微镜、活/死染色、三磷酸腺苷（ATP）测定、菌落形成单位（CFU）计数和定量聚合酶链反应对生物膜进行评估。采用曼-惠特尼U检验比较各组结果。使用电子探针微分析仪评估离子掺入情况。SDF + KI组中的相对ATP含量显著高于SDF组（P < 0.05）。然而，各组之间生物膜形态以及CFU和细菌DNA的对数减少情况相当。与单独使用SDF相比，SDF + KI处理导致的银和氟离子掺入较少。尽管应用KI影响了离子掺入，但SDF和SDF + KI对混合菌种生物膜形成的抑制作用几乎相当。
