微小RNA-129-5p通过下调长链非编码RNA Lnc712抑制人骨肉瘤细胞增殖。
MiR-129-5p Suppresses Cell Proliferation of Human Osteosarcoma Cancer by Down-Regulating LncRNA Lnc712.
作者信息
Yu Yifan, Zuo Wenshan, Cai Wei, Xu Yong, Liu Weidong, Zhao Zexue
机构信息
Department of Orthopaedics, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an City, Nanjing Province, 223300, People's Republic of China.
出版信息
Cancer Manag Res. 2021 Mar 10;13:2259-2264. doi: 10.2147/CMAR.S284078. eCollection 2021.
INTRODUCTION
Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).
METHODS
OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.
RESULTS
Lnc712 was upregulated in OS and was inversely correlated with miR-129-5p. In OS cells, Lnc712 overexpression failed to significantly affect miR-129-5p, while miR-129-5p overexpression led to downregulated Lnc712. Cell proliferation showed that Lnc712 overexpression resulted in increased cell proliferation rate. MiR-129-5p overexpression played an opposite role and reversed the effect of Lnc712 overexpression.
DISCUSSION
MiR-129-5p may suppress cell proliferation of OS by down-regulating Lnc712.
引言
Lnc712已被证实为乳腺癌中的一种致癌长链非编码RNA。本研究旨在探究Lnc712在骨肉瘤（OS）中的作用。
方法
收集58例骨肉瘤患者的骨肉瘤组织及配对的非肿瘤组织。采用RT-qPCR检测配对组织样本中Lnc712和miR-129-5p的表达。在骨肉瘤细胞中实现Lnc712和miR-129-5p表达，以研究它们之间的相互作用。通过CCK-8法分析细胞增殖情况。
结果
Lnc712在骨肉瘤中上调，且与miR-129-5p呈负相关。在骨肉瘤细胞中，Lnc712过表达对miR-129-5p无显著影响，而miR-129-5p过表达导致Lnc712下调。细胞增殖实验表明，Lnc712过表达导致细胞增殖率增加。miR-129-5p过表达则起相反作用，逆转了Lnc712过表达的影响。
讨论
miR-129-5p可能通过下调Lnc712抑制骨肉瘤细胞增殖。
Zotero 插件