Dixit Snehil, Ahmad Irfan, Gular Kumar, Eid Refaat A, Reddy Ravi Shankar, Ribeiro Ivana Leão, Abohashrh Mohammed, Alshahrani Mastour Saeed, Tedla Jaya Shanker, Dixit Nitin Arun
Department of Medical Rehabilitation Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Saudi J Biol Sci. 2021 Mar;28(3):1678-1686. doi: 10.1016/j.sjbs.2020.12.004. Epub 2020 Dec 8.
The primary purpose of the recent experiment was to scrutinize the dissimilarity between single and multiple exposures by electrotherapeutic modalities to determine the development of Gram-positive and Gram negative bacteria spectrum.
Bacterial strains employed in this study were Gram-negative bacteria such as and and Gram-positive bacteria such as and Fluence for Low level laser therapy (LLLT) (810 nm) was 40 J/cm for 80 s, for microwave (MWD) a dosage of 100-Watt with duration of 5 min and for magnetic field therapy (MT) duration of 30 min with 100% intensity was used.
Repeated Measures of analysis of variances (RANOVA) for within-subject effects was used to detect a global significant change within the means at dissimilar time points. The experiments of within-subjects revealed a significant difference within groups, df of (3, 40), F value of 39.38 and a p value less than 0.001, representing a significant variation between the three groups between pre and post exposures. There was a significant variation between single exposure and multiple exposures in the experimental sample's pre-post between the four groups with df (1, 40) f value of 2943.69 and p value less than 0.001. Scanning and Transmission electron microscopy images were also taken into account to determine the extent of damage caused to the bacterial cells surface topography in Gram negative and Gram positive spectrums.
The study demonstrated that single high exposure with the LLLT appears to have the most emphatic effect followed by exposure by MWD and MT.
近期实验的主要目的是仔细研究通过电疗方式进行单次和多次暴露之间的差异，以确定革兰氏阳性菌和革兰氏阴性菌谱的发展情况。
本研究中使用的细菌菌株为革兰氏阴性菌，如……和……，以及革兰氏阳性菌，如……和……。低强度激光疗法（LLLT）（810nm）的能量密度为40J/cm²，持续80秒；微波疗法（MWD）的剂量为100瓦，持续5分钟；磁场疗法（MT）的持续时间为30分钟，强度为100%。
采用重复测量方差分析（RANOVA）来检测不同时间点均值之间的总体显著变化。组内实验显示组内存在显著差异，自由度为（3,40），F值为39.38，p值小于0.001，表明三组在暴露前后存在显著差异。在四个组的实验样本暴露前后，单次暴露和多次暴露之间存在显著差异，自由度为（1,40），f值为2943.69，p值小于0.001。还考虑了扫描电子显微镜和透射电子显微镜图像，以确定革兰氏阴性菌和革兰氏阳性菌谱中细菌细胞表面形貌的损伤程度。
该研究表明，单次高剂量的LLLT暴露似乎具有最显著的效果，其次是MWD和MT暴露。
