Xu Jing, Su Guannan, Huang Xinyue, Chang Rui, Chen Zhijun, Ye Zi, Cao Qingfeng, Kijlstra Aize, Yang Peizeng
The First Affiliated Hospital of Chongqing Medical University, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, China.
University Eye Clinic Maastricht, Maastricht, Netherlands.
Front Immunol. 2021 Feb 22;12:587393. doi: 10.3389/fimmu.2021.587393. eCollection 2021.
To investigate aqueous metabolic profiles in Vogt-Koyanagi-Harada (VKH) and Behcet's disease (BD), we applied ultra-high-performance liquid chromatography equipped with quadrupole time-of flight mass spectrometry in aqueous humor samples collected from these patients and controls. Metabolite levels in these three groups were analyzed by univariate logistic regression. The differential metabolites were subjected to subsequent pathway analysis by MetaboAnalyst. The results showed that both partial-least squares discrimination analysis and hierarchical clustering analysis showed specific aqueous metabolite profiles when comparing VKH, BD, and controls. There were 28 differential metabolites in VKH compared to controls and 29 differential metabolites in BD compared to controls. Amino acids and fatty acids were the two most abundant categories of differential metabolites. Furthermore, pathway enrichment analysis identified several perturbed pathways, including pantothenate and CoA biosynthesis when comparing VKH with the control group, and D-arginine and D-ornithine metabolism and phenylalanine metabolism when comparing BD with the control group. Aminoacyl-tRNA biosynthesis was altered in both VKH and BD when compared to controls. Our findings suggest that amino acids metabolism as well as two fatty acids, palmitic acid and oleic acid, may be involved in the pathogenesis of BD and VKH.
为了研究小柳原田病（VKH）和白塞病（BD）的房水代谢谱，我们将配备四极杆飞行时间质谱仪的超高效液相色谱应用于从这些患者及对照组采集的房水样本。通过单因素逻辑回归分析这三组中的代谢物水平。对差异代谢物进行MetaboAnalyst后续通路分析。结果显示，在比较VKH、BD和对照组时，偏最小二乘判别分析和层次聚类分析均显示出特定的房水代谢物谱。与对照组相比，VKH中有28种差异代谢物，BD中有29种差异代谢物。氨基酸和脂肪酸是差异代谢物中最丰富类别中的两类。此外，通路富集分析确定了几条受干扰的通路，在比较VKH与对照组时包括泛酸和辅酶A生物合成，在比较BD与对照组时包括D - 精氨酸和D - 鸟氨酸代谢以及苯丙氨酸代谢。与对照组相比，VKH和BD中氨酰 - tRNA生物合成均发生改变。我们的研究结果表明，氨基酸代谢以及两种脂肪酸，即棕榈酸和油酸，可能参与了BD和VKH的发病机制。
