Xin Zengxi, Tong Zhou, Tan Jingyu, Liu Changfu
Department of Prosthodontics, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China.
Exp Ther Med. 2021 Apr;21(4):373. doi: 10.3892/etm.2021.9804. Epub 2021 Feb 19.
MicroRNA-145-5p (miR-145-5p) is expressed in a variety of tumors, but the mechanism underlying miR-145-5p in tongue squamous cell carcinoma (TSCC) is not fully understood. Therefore, the present study investigated the role of miR-145-5p in TSCC. miR-145-5p expression levels in TSCC tissues were analyzed via reverse transcription-quantitative PCR. miR-145-5p mimics and inhibitors were transfected into SCC9 and Cal27 cells. The stability and invasion of SCC9 and Cal27 cells were analyzed by performing Transwell assays, while PI and Annexin V were used to detect cell apoptosis. Oxidative stress levels of superoxide dismutase, malondialdehyde and glutathione peroxidase were measured via ELISA. PI3K/AKT signaling pathway-associated protein expression levels were evaluated using western blotting. miR-145-5p was consistently downregulated in TSCC tissues compared with healthy tissues. miR-145-5p overexpression decreased cell stability and invasion, but promoted cell apoptosis and oxidative stress. In addition, PI3K, AKT and phosphorylated-AKT expression levels were significantly diminished. The results indicated that miR-145-5p overexpression inhibited SCC9 and Cal27 cell stability and invasion, promoted SCC9 and Cal27 cell apoptosis and oxidative stress, and inhibited the PI3K/AKT signaling pathway. The results of the present study suggested that miR-145 may serve as a molecular marker of TSCC.
微小RNA-145-5p（miR-145-5p）在多种肿瘤中均有表达，但miR-145-5p在舌鳞状细胞癌（TSCC）中的潜在机制尚未完全明确。因此，本研究探讨了miR-145-5p在TSCC中的作用。通过逆转录定量聚合酶链反应分析TSCC组织中miR-145-5p的表达水平。将miR-145-5p模拟物和抑制剂转染至SCC9和Cal27细胞中。通过Transwell实验分析SCC9和Cal27细胞的稳定性和侵袭能力，同时使用碘化丙啶（PI）和膜联蛋白V检测细胞凋亡情况。通过酶联免疫吸附测定法检测超氧化物歧化酶、丙二醛和谷胱甘肽过氧化物酶的氧化应激水平。使用蛋白质免疫印迹法评估PI3K/AKT信号通路相关蛋白的表达水平。与健康组织相比，TSCC组织中miR-145-5p的表达持续下调。miR-145-5p过表达降低了细胞稳定性和侵袭能力，但促进了细胞凋亡和氧化应激。此外，PI3K、AKT和磷酸化-AKT的表达水平显著降低。结果表明，miR-145-5p过表达抑制了SCC9和Cal27细胞的稳定性和侵袭能力，促进了SCC9和Cal27细胞的凋亡和氧化应激，并抑制了PI3K/AKT信号通路。本研究结果提示，miR-145可能作为TSCC的分子标志物。
