Kim Min-Ah, Lee Hyun-Jong, Bae Hyun-Gyeong, Yang Seung-Ok, Lee Hee Jae, Kim Mi-Ja
Department of Food and Nutrition, College of Health Science, Kangwon National University, Samcheok, Gangwon 25949 Republic of Korea.
National Instrumentation Center for Environmental Management, Seoul National University, Seoul, 08826 Republic of Korea.
Food Sci Biotechnol. 2021 Feb 23;30(2):277-286. doi: 10.1007/s10068-020-00866-9. eCollection 2021 Feb.
Celery seeds were fractioned successively by organic solvents, and fractions were screened for their antioxidant properties. Metabolites of high antioxidative fractions were analyzed, and then anti-obesity properties and mechanisms of selected fractions were evaluated in 3T3-L1 adipocytes. Celery seed ethanol extract (CSE) and celery seed ethyl acetate fraction (CSEA) possessed higher in vitro antioxidant properties, including radical scavenging and total phenolic content, than that of hexane, butanol, and water fractions. Total of 43 and 19 metabolites were identified from CSE and CSEA, respectively, by gas chromatography-mass selective detector. Isoferulic acid and -coumaric acid were only found in CSEA. CSEA possessed higher anti-adipogenesis in the 3T3-L1 cells than CSE by decreasing adipogenic hormones, including leptin and adiponectin. Expression levels of adipocyte-related transcription factors and genes such as , , , and decreased to comparable levels of control. CSEA could be used as a natural ingredient with antioxidant and anti-obesity functionality.
芹菜籽先用有机溶剂进行连续分级，然后对各分级进行抗氧化性能筛选。分析了高抗氧化分级的代谢产物，随后在3T3-L1脂肪细胞中评估了所选分级的抗肥胖特性及作用机制。芹菜籽乙醇提取物（CSE）和芹菜籽乙酸乙酯分级（CSEA）在体外具有比己烷、丁醇和水分级更高的抗氧化性能，包括自由基清除能力和总酚含量。通过气相色谱-质量选择性检测器分别从CSE和CSEA中鉴定出43种和19种代谢产物。异阿魏酸和对香豆酸仅在CSEA中发现。CSEA通过降低包括瘦素和脂联素在内的脂肪生成激素，在3T3-L1细胞中具有比CSE更高的抗脂肪生成作用。脂肪细胞相关转录因子和基因如 、 、 和 的表达水平降至与对照相当的水平。CSEA可作为一种具有抗氧化和抗肥胖功能的天然成分。
