牛至籽代谢产物及其通过下调脂肪生成对3T3-L1脂肪细胞的抗肥胖作用。
Metabolites of oregano () seed and their anti-obesity effects on 3T3-L1 adipocytes through down-regulated adipogenesis.
作者信息
Lee Hyun-Jong, Kim Mi-Ja
机构信息
Department of Food and Nutrition, College of Health Science, Kangwon National University, Samcheok, Gangwon 25949 Korea.
出版信息
Food Sci Biotechnol. 2022 Apr 20;31(7):867-878. doi: 10.1007/s10068-022-01085-0. eCollection 2022 Jul.
Metabolites of the 80% ethanol extract (OSE) and ethyl acetate fraction (OSEA) of oregano seed were analyzed by GC-MS, and anti-obesity effects of OSE and OSEA were evaluated in 3T3-L1 adipocyte. OSE possessed high content of glucose, fructofuranose, and sucrose while OSEA had high content of phenolic chemicals. OSEA contained higher levels of gallic acid, syringic acid, protocatechuic acid, and catechin than OSE. OSEA inhibited lipid droplet accumulation with concentration dependent manner in 3T3-L1 preadipocytes during differentiation. OSEA showed more inhibition ability than OSE by 13.7-fold at the level of 125 μg/mL. Additionally, relative mRNA and protein expression levels of , , , and which are related to adipogenesis were significantly lower in OSEA treatment group than in OSE treatment group ( < 0.05). Therefore, OSEA could be used as anti-obesity functional ingredient.
采用气相色谱-质谱联用仪（GC-MS）分析了牛至种子80%乙醇提取物（OSE）和乙酸乙酯部位（OSEA）的代谢产物，并在3T3-L1脂肪细胞中评估了OSE和OSEA的抗肥胖作用。OSE含有高含量的葡萄糖、呋喃果糖和蔗糖，而OSEA含有高含量的酚类化合物。与OSE相比，OSEA含有更高水平的没食子酸、丁香酸、原儿茶酸和儿茶素。在分化过程中，OSEA以浓度依赖的方式抑制3T3-L1前脂肪细胞中脂滴的积累。在125μg/mL水平时，OSEA的抑制能力比OSE高13.7倍。此外，与脂肪生成相关的 、 、 和 的相对mRNA和蛋白表达水平在OSEA处理组中显著低于OSE处理组（ ＜0.05）。因此，OSEA可作为抗肥胖功能成分。
Zotero 插件