鲁辛朵和4P-PDOT根据其浓度阻断褪黑素对牛颗粒细胞凋亡和细胞周期的影响。
Luzindole and 4P-PDOT block the effect of melatonin on bovine granulosa cell apoptosis and cell cycle depending on its concentration.
作者信息
Wang Shujuan, Liu Wenju, Wen Aiyou, Yang Bing, Pang Xunsheng
机构信息
College of Animal Science, Anhui Science and Technology University, Fengyang, China.
Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang, China.
出版信息
PeerJ. 2021 Mar 8;9:e10627. doi: 10.7717/peerj.10627. eCollection 2021.
Granulosa cells play an essential physiological role in mediating the follicle development and survival or apoptosis of granulosa cells dictate the follicle development or atresia. The aim of this study was to investigate the role of high dose (10 M) and low dose (10 M) melatonin in bovine granulosa cells, and assess whether MT1 and MT2 inhibiter affect granulosa cells response to melatonin. We found that the high dose (10 M) and low dose (10 M) both could act as an essential role in modulating granulosa cells apoptosis, cell cycle and antioxidant. The beneficial effect could be related to that melatonin promoted the expression of , , and , and inhibited , and expression. Moreover expression was decreased in granulosa cells treated with the high dose (10 M) melatonin and increased in that treated with the low dose (10 M) melatonin. To further reveal the role of MT1 and MT2 in mediating the effect of melatonin on granulosa cells apoptosis, cell cycle and antioxidant, we found that the luzindole and 4P-PDOT did not affect the effect of high dose (10 M) melatonin on regulating , , , , and expression, while blocked its effect on modulating and expression. However, luzindole and 4P-PDOT disturbed the effect of low dose (10 M) melatonin on regulating , , , , , , and expression. In conclusion, these results reveal that the effect of low dose (10 M) melatonin on granulosa cells apoptosis are mediated by MT1 and MT2, and the high dose (10 M) melatonin affect the granulosa cells apoptosis by other pathway, besides MT1 and MT2. Moreover MT1 and MT2 may work in concert to modulate bovine granulosa cells function by regulating cellular progression and apoptosis.
颗粒细胞在介导卵泡发育过程中发挥着重要的生理作用，颗粒细胞的存活或凋亡决定了卵泡的发育或闭锁。本研究的目的是探讨高剂量（10 μM）和低剂量（10 nM）褪黑素在牛颗粒细胞中的作用，并评估MT1和MT2抑制剂是否影响颗粒细胞对褪黑素的反应。我们发现高剂量（10 μM）和低剂量（10 nM）褪黑素在调节颗粒细胞凋亡、细胞周期和抗氧化方面均发挥着重要作用。这种有益作用可能与褪黑素促进 、 、 和 的表达，以及抑制 、 和 的表达有关。此外，高剂量（10 μM）褪黑素处理的颗粒细胞中 表达降低，而低剂量（10 nM）褪黑素处理的颗粒细胞中 表达增加。为了进一步揭示MT1和MT2在介导褪黑素对颗粒细胞凋亡、细胞周期和抗氧化作用中的作用，我们发现鲁辛朵和4P-PDOT不影响高剂量（10 μM）褪黑素对 、 、 、 、 和 表达的调节作用，但阻断了其对 和 表达的调节作用。然而，鲁辛朵和4P-PDOT干扰了低剂量（10 nM）褪黑素对 、 、 、 、 、 和 表达的调节作用。总之，这些结果表明低剂量（10 nM）褪黑素对颗粒细胞凋亡的作用是由MT1和MT2介导的，而高剂量（10 μM）褪黑素除了MT1和MT2外，还通过其他途径影响颗粒细胞凋亡。此外，MT1和MT2可能协同作用，通过调节细胞进程和凋亡来调节牛颗粒细胞的功能。
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