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褪黑素及其受体 MT2 在牛颗粒细胞发育中的作用机制。

The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells.

机构信息

College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China.

Research Center for Biomedical and Health Science, Anhui Science and Technology University, Fengyang 233100, China.

出版信息

Int J Mol Sci. 2018 Jul 12;19(7):2028. doi: 10.3390/ijms19072028.

DOI:10.3390/ijms19072028
PMID:30002300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6073438/
Abstract

Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of in bovine GCs, and assess whether silencing affected GCs response to melatonin. We found that silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of silencing on steroids synthesis, it was found that the expression of and enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (, , and ) were up-regulated without affecting the expression of , suggesting that silencing may regulate hormone abundance. Furthermore, silencing significantly increased the expression of and , and decreased the expression of and without significant difference in the expression of and . In addition, silencing didn't affect the effect of melatonin on increasing the expression of , , and , and progesterone level, or decreasing , and expression, and production of inhibin B. Moreover, silencing could disrupt the role of melatonin in decreasing the , and expression, and activin B secretion. In conclusion, these results reveal that melatonin and MT2 are essential regulator of bovine GCs function by modulating reproduction-related genes expression, hormones secretion and other regulators of folliculogenesis.

摘要

卵巢颗粒细胞 (GCs) 是研究卵泡发生过程中基因调控机制的重要方法。本研究旨在探讨在牛 GCs 中的作用，并评估沉默是否影响 GCs 对褪黑素的反应。我们发现，沉默显著降低了孕激素和雌二醇的分泌，增加了抑制素 B 和激活素 B 的浓度。为了进一步揭示沉默对类固醇合成的调节机制，发现 和 酶（类固醇激素合成）的表达下调，而与激素合成相关的基因（ 、 、 和 ）上调，而不影响 的表达，表明沉默可能调节激素丰度。此外，沉默显著增加了 和 的表达，降低了 和 的表达，而 和 的表达没有显著差异。此外，沉默不影响褪黑素对增加 、 、 和孕激素水平的作用，或降低 、 和 的表达和抑制素 B 的分泌。此外，沉默可以破坏褪黑素在降低 、 和 表达和激活素 B 分泌中的作用。总之，这些结果表明，褪黑素和 MT2 通过调节与生殖相关的基因表达、激素分泌和其他卵泡发生调节剂来调节牛 GCs 的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/0792d3cea5da/ijms-19-02028-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/51eecedf5f52/ijms-19-02028-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/0792d3cea5da/ijms-19-02028-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/51eecedf5f52/ijms-19-02028-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/d4ea035b900d/ijms-19-02028-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/2854e7f8833b/ijms-19-02028-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/d2536b3744ef/ijms-19-02028-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f97/6073438/0792d3cea5da/ijms-19-02028-g005.jpg

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