Botero Leidy, Vizcaíno Samuel, Quiñones Winston, Echeverri Fernando, Gil Jesús, Durango Diego
Universidad Nacional de Colombia-Sede Medellín, Facultad de Ciencias, Escuela de Química, Carrera 65, 59ª-110, Medellín, Colombia.
Química Orgánica de Productos Naturales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Calle 70 N° 52-21, P.O. Box 1226, Medellín, Colombia.
Biotechnol Rep (Amst). 2021 Feb 23;29:e00601. doi: 10.1016/j.btre.2021.e00601. eCollection 2021 Mar.
Isoflavonoid phytoalexins (isoflavones: genistein, 2'-hydroxygenistein, and daidzein; isoflavanones: dalbergioidin and kievitone; coumestrol; pterocarpans: phaseollidin and phaseollin; and the isoflavan: phaseollinisoflavan) production in response to the application of eleven 1-oxo-indane-4-carboxylic acid derivatives (indanoyl esters and indanoyl amino acids conjugates), in cotyledons and hypocotyl/root of two common bean ( L.) cultivars was evaluated. The content of isoflavonoids depended on the cultivar, the treated tissue, the time after induction, the structure and concentration of the elicitor. The highest isoflavonoid contents were found when 1-oxo-indanoyl-amino acids conjugates were used as elicitors. Cotyledons and hypocotyl/root of the anthracnose-resistant cultivar produced significantly higher isoflavonoid contents as compared to the susceptible one. Maximum levels of phaseollin were obtained using 0.66 mM 1-oxo-indanoyl-l-isoleucyl methyl ester and between 72 and 96 h post-induction. So, 1-oxo-indane-4-carboxylic acid derivatives, may be used to enhance the amount of isoflavonoid phytoalexins in common bean and protect crops from phytopathogenic microorganisms.
评估了两种菜豆（L.）品种的子叶和下胚轴/根中，异黄酮类植保素（异黄酮：染料木黄酮、2'-羟基染料木黄酮和大豆苷元；异黄烷酮：黄檀素和基维酮；香豆雌酚；紫檀素：菜豆素和菜豆灵；以及异黄烷：菜豆异黄烷）对十一种1-氧代茚满-4-羧酸衍生物（茚满酰酯和茚满酰氨基酸共轭物）处理的响应情况。异黄酮的含量取决于品种、处理的组织、诱导后的时间、激发子的结构和浓度。当使用1-氧代茚满酰氨基酸共轭物作为激发子时，异黄酮含量最高。与感病品种相比，抗炭疽病品种的子叶和下胚轴/根产生的异黄酮含量显著更高。使用0.66 mM 1-氧代茚满酰-L-异亮氨酸甲酯并在诱导后72至96小时之间可获得菜豆灵的最大含量。因此，1-氧代茚满-4-羧酸衍生物可用于提高菜豆中异黄酮类植保素的含量，并保护作物免受植物病原微生物的侵害。
