Sorokin Aleksei, Yadav Narendra Singh, Gaudet Daniel, Kovalchuk Igor
Department of Biological Sciences, University of Lethbridge, Lethbridge T1K 3M4, Alberta, Canada.
Bio Protoc. 2021 Jan 5;11(1):e3875. doi: 10.21769/BioProtoc.3875.
Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for using a hydrogen peroxide (HO) solution as liquid germination media. In this protocol, all three steps including seed sterilization, germination, and seedlings development were carried out in an HO solution of different concentrations; 1% HO solution showed the fastest and the most efficient germination. This protocol also exhibited high germination efficiency for very old cannabis seeds with lower viability. Overall, this protocol demonstrates superior germination compared to water control and reduces the risk of contamination, making it suitable for tissue culture and other sensitive applications.
大麻种子萌发对种植者和研究人员来说都是一个重要过程。许多生物技术应用都需要一种可靠的种子无菌萌发方法。本方案概述了一种使用过氧化氢（H₂O₂）溶液作为液体萌发培养基的种子萌发程序。在本方案中，包括种子灭菌、萌发和幼苗发育在内的所有三个步骤均在不同浓度的H₂O₂溶液中进行；1%的H₂O₂溶液显示出最快且最有效的萌发效果。该方案对活力较低的非常陈旧的大麻种子也表现出高萌发效率。总体而言，与水对照相比，该方案显示出卓越的萌发效果，并降低了污染风险，使其适用于组织培养和其他敏感应用。
