Pooranachithra Murugesan, Bhaskar James Prabhanand, Balamurugan Krishnaswamy
Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India.
ITC - Life Sciences and Technology Centre, Peenya Industrial Area, 1st Phase, Bangalore, Karnataka, India.
Bio Protoc. 2021 Jan 20;11(2):e3885. doi: 10.21769/BioProtoc.3885.
Research on wound healing majorly relies on rat, mice and other animal models. However, an alternative animal model ought to be brought in the field, pertaining to the stringent ethical issues owing to the use of animals in research. In this regard, , a miniature model nematode gains the great attention of the researchers in wound healing. Though, the model is being explored in wound research for more than a decade, the existing protocols lack the acquisition of large wound population that in turn could enable the utility of global genomics (G), proteomics (P) and metabolomics (M) based approaches. In order to overcome the inadequacy of the existing protocols, the protocol described here affords the acquisition of voluminous wound population in using truncated glasswool pieces to enable the utility of high throughput analytical techniques.
伤口愈合的研究主要依赖于大鼠、小鼠和其他动物模型。然而，由于在研究中使用动物存在严格的伦理问题，该领域应引入替代动物模型。在这方面，一种小型线虫模型引起了伤口愈合研究人员的极大关注。尽管该模型在伤口研究中已被探索了十多年，但现有方案缺乏获取大量伤口群体的方法，而这反过来又能使基于全球基因组学（G）、蛋白质组学（P）和代谢组学（M）的方法得以应用。为了克服现有方案的不足，本文所述的方案通过使用截断的玻璃棉片在[具体实验对象]中获取大量伤口群体，以实现高通量分析技术的应用。
