Hindie E, Colas-Linhart N, Petiet A, Bok B
Department of Biophysics, Faculty of Medecine X. Bichat, Paris, France.
J Nucl Med. 1988 Jun;29(6):1118-21.
A new microautoradiographic technique was developed to study the distribution of 99mTc-labeled radiopharmaceuticals. Using a thick emulsion, it is possible to get microscopically visible tracks of internal conversion and Auger electrons. The liver uptake of microscopic particles has been thought to occur in Kupffer cells but no direct evidence has been provided for technetium colloids. Using this method, 99mTc-labeled colloids were clearly identified in Kupffer cells in the sinusoidal areas of liver. "Track" microautoradiography using a thick emulsion layer may be used on any frozen tissue sections and may provide an important tool to assess the biodistribution of 99mTc radiopharmaceuticals.
一种新的显微放射自显影技术被开发用于研究99mTc标记的放射性药物的分布。使用厚乳胶，可以获得内转换电子和俄歇电子在显微镜下可见的径迹。肝脏对微观颗粒的摄取一直被认为发生在库普弗细胞中，但对于锝胶体尚未提供直接证据。使用这种方法，在肝脏窦状隙区域的库普弗细胞中清楚地识别出了99mTc标记的胶体。使用厚乳胶层的“径迹”显微放射自显影可用于任何冷冻组织切片，并可能为评估99mTc放射性药物的生物分布提供一种重要工具。
