Puncher M R, Blower P J
Biological Laboratory, University of Kent at Canterbury, UK.
Eur J Nucl Med. 1994 Dec;21(12):1347-65. doi: 10.1007/BF02426701.
The understanding of localisation mechanisms and microdosimetry of diagnostic and therapeutic radiopharmaceuticals depends on knowledge of their biodistribution at the microscopic level (cellular and subcellular) in the target tissues. Various methods have been advanced for obtaining information about this microdistribution: subcellular fractionation, secondary ion mass spectrometry imaging, microprobe elemental analysis in the electron microscope, and microautoradiography. This review compares these approaches, and discusses in detail the methodology of microautoradiography (the most generally useful approach) with imaging and therapy radionuclides. Literature examples of applications of microautoradiography in nuclear medicine are reviewed, and the future potential contribution of the techniques is assessed.
对诊断和治疗性放射性药物的定位机制和微剂量学的理解取决于对其在靶组织微观水平（细胞和亚细胞水平）生物分布的了解。已经提出了各种方法来获取有关这种微观分布的信息：亚细胞分级分离、二次离子质谱成像、电子显微镜中的微探针元素分析以及微放射自显影。本综述比较了这些方法，并详细讨论了使用成像和治疗放射性核素的微放射自显影方法（最普遍有用的方法）。回顾了微放射自显影在核医学中应用的文献实例，并评估了该技术未来的潜在贡献。
