Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
STAR Protoc. 2021 Mar 5;2(1):100371. doi: 10.1016/j.xpro.2021.100371. eCollection 2021 Mar 19.
The maintenance of stem cell populations and the differentiation of their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging of the ovarian germline stem cell niche By immobilizing the ovarian tissue in a fibrinogen-thrombin clot, we are able to maintain the tissue for short-term high-temporal live imaging. This enables the visualization of dynamic cellular processes, such as the cytoskeletal dynamics that control stem cell niche communication. For complete details on the use and execution of this protocol, please refer to Wilcockson and Ashe (2019).
干细胞群体的维持和其后代的分化是通过与相关龛细胞的特定通信来协调的。在这里，我们描述了一种短期活体成像卵巢生殖干细胞龛的方案。通过将卵巢组织固定在纤维蛋白原-凝血酶凝块中，我们能够在短期内进行高时间分辨率的活体成像。这使得可视化动态细胞过程成为可能，例如控制干细胞龛通信的细胞骨架动力学。有关此协议的使用和执行的完整详细信息，请参阅 Wilcockson 和 Ashe（2019 年）。
