Area of Helminthology, CENID-Animal Health and Safety, INIFAP, Jiutepec, Mexico.
Tropical Mushroom Laboratory, Southern Frontier College, Tapachula, Mexico.
J Med Food. 2021 Sep;24(9):953-959. doi: 10.1089/jmf.2020.0168. Epub 2021 Mar 17.
During the previous decades, the indiscriminate use of anthelmintics for the control of gastrointestinal nematodes has generated anthelmintic resistance in different parts of the world. It is necessary to search for new sustainable control alternatives, such as the use of extracts from plants and edible mushrooms. Therefore, the aim of the present study was to evaluate the nematicidal activity of extracts and fractions of the edible mushroom against eggs and infective larvae. The basidiomata of ECS-401 were provided by the Tropical Fungi Laboratory of El Colegio de la Frontera Sur and were extracted with ethyl acetate, methanol, and water. Fractionation of the most active extract was carried out by open column chromatography. The bioassays were performed in 96-well microtiter plates using 100 eggs/larvae, a final volume of 100 μL, and different concentrations of extracts/fractions ( = 4). Bioassay readings were taken at 48 h for egg hatching inhibition (EHI) and at 24, 48 and 72 h for larval mortality (LM). The ethyl acetate extract exhibited the highest percentage of EHI (100%). For the LM bioassay, the aqueous extract was the most active (69%), but its fractions did not show larvicidal activity. The chemical profile of the aqueous extract was analyzed by high-performance liquid chromatography, which showed the presence of phenols, flavonoids, and terpenes. extracts showed ovicidal and/or larvicidal activity.
在过去的几十年里，人们为了控制胃肠道线虫而不加选择地使用驱虫药，这导致了世界各地驱虫药的耐药性。因此，有必要寻找新的可持续控制替代方案，例如使用植物和可食用蘑菇的提取物。因此，本研究的目的是评估可食用蘑菇对鸡蛋和感染性幼虫的杀线虫活性。ECS-401 的担子果由热带真菌实验室 El Colegio de la Frontera Sur 提供，并用乙酸乙酯、甲醇和水提取。最活跃的提取物通过开放柱色谱进行了分级。使用 100 个鸡蛋/幼虫、终体积为 100 μL 和不同浓度的提取物/馏分（ = 4）在 96 孔微量滴定板上进行了生物测定。孵育 48 小时以抑制卵孵化（EHI），24、48 和 72 小时以测量幼虫死亡率（LM）。乙酸乙酯提取物表现出最高的 EHI（100%）。对于 LM 生物测定，水提取物最有效（69%），但其馏分没有显示出杀幼虫活性。通过高效液相色谱分析了水提取物的化学特征，结果表明存在酚类、类黄酮和萜类化合物。这些提取物表现出杀卵和/或杀幼虫活性。
