González-Cortázar Manases, Sánchez José E, Huicochea-Medina Magaly, Hernández-Velázquez Victor M, Mendoza-de-Gives Pedro, Zamilpa Alejandro, López-Arellano Ma Eugenia, Pineda-Alegría Jesús Antonio, Aguilar-Marcelino Liliana
Southern Biomedical Research Center, Mexican Institute of Social Security, Xochitepec, Morelos, Mexico.
Tropical Mushroom Laboratory, Southern Frontier College, Tapachula, Chiapas, Mexico.
J Med Food. 2021 Mar;24(3):310-318. doi: 10.1089/jmf.2020.0054. Epub 2020 Jul 27.
Sheep haemonchosis is a disease that causes anemia, diarrhea, weight loss, low production, and even death of young animals. This represents a negative economic impact on the livestock sector. For decades, chemicals have been used to control this parasitic disease; however, cases of anthelmintic resistance have increased around the world. For this reason, the search for control alternatives is necessary. Several studies have shown that edible mushrooms of genus have different medicinal properties, including nematicidal activity. In this study, the chromatographic fractionation of the hydroalcoholic extract of fruiting bodies was carried out to identify and isolate the metabolites responsible for nematicidal activity. From the fractionation of the extract, the fraction PdR2 and the subfraction PdB were obtained, which were evaluated against under conditions. The nematicidal effect of the fraction PdB in gerbils infected artificially with was evaluated. Finally, the analysis of the PdB fraction by gas chromatography-mass spectrometry and nuclear magnetic resonance was carried out. The results showed that the PdB had 100% of egg hatching inhibition from 5 mg/mL. Regarding larvicidal activity, it presented >97.2% from 24 h to 20 mg/mL. The evaluation of the PdB fraction showed a reduction of larvae of 92.56%. The compounds present in this fraction were the mixture of allitol and an unidentified terpene in a ratio of 9:1. The PdB fraction is a potential alternative for the control of , where allitol and a terpene could be responsible for nematicidal activity.
绵羊捻转血矛线虫病是一种可导致贫血、腹泻、体重减轻、生产性能低下甚至幼畜死亡的疾病。这对畜牧业造成了负面经济影响。几十年来，化学药物一直被用于控制这种寄生虫病；然而，世界各地抗驱虫药的病例有所增加。因此，有必要寻找其他控制方法。多项研究表明，某些属的食用菌具有不同的药用特性，包括杀线虫活性。在本研究中，对子实体的水醇提取物进行色谱分离，以鉴定和分离具有杀线虫活性的代谢物。通过提取物的分离，获得了组分PdR2和亚组分PdB，并在特定条件下对其进行了评估。评估了亚组分PdB对人工感染某种线虫的沙鼠的杀线虫效果。最后，通过气相色谱-质谱联用和核磁共振对PdB组分进行了分析。结果表明，PdB在5mg/mL时对虫卵孵化的抑制率为100%。在杀幼虫活性方面，在24小时至20mg/mL的浓度范围内，其杀幼虫活性大于97.2%。对PdB组分的评估显示，线虫幼虫数量减少了92.56%。该组分中的化合物是阿糖醇和一种未鉴定的萜烯的混合物，比例为9:1。PdB组分是控制某种线虫的潜在替代方法，其中阿糖醇和一种萜烯可能是杀线虫活性的原因。
