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从圈养果蝠中分离出的新物种和新物种的封闭基因组序列。

Closed genome sequences of sp. nov. and sp. nov. isolated from captive fruit bats ().

作者信息

Fountain Kay, Gibbon Marjorie J, Loeffler Anette, Feil Edward J

机构信息

Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.

Department of Clinical Science and Services, Royal Veterinary College, Hatfield, North Mymms, Hertfordshire, AL9 7TA, UK.

出版信息

Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004715. Epub 2021 Mar 18.

Abstract

The increasing availability of whole genome sequencing of bacteria has accelerated the discovery of novel species which may not have been easy to discriminate using standard phenotypic or single gene methods. Phylogenomic analysis of genome sequences from a collection of coagulase-negative staphylococcal species isolated from captive fruit bats revealed two clusters which were close to . To assess the relatedness of the strains we used digital DNA-DNA hybridization (dDDH) and two methods for average nucleotide identity (ANI) computation which predicted two novel species having dDDH less than 70 % and ANI less than 95%. We propose these species as sp. nov. (type strain 23_2_7_LY=NCTC 14453=DSM 111639) and sp. nov (type strain 27_4_6_LY=NCTC 14454=DSM 111640).

摘要

细菌全基因组测序的可得性不断提高,加速了新物种的发现,而使用标准表型或单基因方法可能难以区分这些新物种。对从圈养果蝠中分离出的一组凝固酶阴性葡萄球菌物种的基因组序列进行系统发育基因组分析,发现了两个与……接近的簇。为了评估这些菌株的相关性,我们使用了数字DNA-DNA杂交(dDDH)和两种计算平均核苷酸同一性(ANI)的方法,预测出两个新物种,其dDDH小于70%,ANI小于95%。我们将这些物种命名为 sp. nov.(模式菌株23_2_7_LY=NCTC 14453=DSM 111639)和 sp. nov(模式菌株27_4_6_LY=NCTC 14454=DSM 111640)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/8375422/61023400930a/ijsem-71-4715-g001.jpg

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