Volokhov Dmitriy V, Zagorodnyaya Tatiana A, Furtak Vyacheslav A, Nattanmai Geetha, Randall Linnell, Jose Sherly, Gao Yamei, Eisenberg Tobias, Delmonte Pierluigi, Blom Jochen, Mitchell Kara K
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.
Bacteriology Laboratory, Wadsworth Center, the New York State Department of Health, 120 New Scotland Ave, Albany, NY 12208, USA.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.006015.
One novel strain (SQ9-PEA) and two novel strains (SQ8-PEA and GRT3) were isolated from faeces of a wild eastern grey squirrel. The strains were non-spore-forming, non-motile Gram-positive cocci, facultative anaerobes. The genomes for these strains were sequenced. The 16S rRNA gene and core-genome-based phylogenetic analyses showed that strain SQ9-PEA was closely related to , strain SQ8-PEA to and , and strain GRT3 to , and . Average nucleotide identity and pairwise digital DNA-DNA hybridization values calculated for these novel strains compared to type strain genomes of phylogenetically related species within the genera and clearly revealed that strain SQ9-PEA represents a novel species of the genus and strains SQ8-PEA and GRT3 represent two novel species of the genus . Phenotypical features of these novel type strains differed from the features of the type strains of other phylogenetically related species. MALDI-TOF mass spectrometry supported identification of these novel species. Based on these data, we propose one novel species of the genus , for which the name sp. nov. with the type strain SQ9-PEA (=DSM 114656=CCUG 76426=NCTC 14727) is proposed, and two novel species of the genus , for which the names sp. nov. with the type strain SQ8-PEA (=DSM 114685=CCUG 76423=NCTC 14723) and sp. nov. with the type strain GRT3 (=DSM 114696=CCUG 76427=NCTC 14722) are proposed. The genome G+C contents are 38.29, 36.49 and 37.26 mol% and complete draft genome sizes are 1 692 266, 2 371 088 and 2 237 001 bp for strains SQ9-PEA, SQ8-PEA and GRT3, respectively.
从一只野生东部灰松鼠的粪便中分离出一株新菌株（SQ9-PEA）和两株新菌株（SQ8-PEA和GRT3）。这些菌株为无芽孢、无运动性的革兰氏阳性球菌，兼性厌氧菌。对这些菌株的基因组进行了测序。基于16S rRNA基因和核心基因组的系统发育分析表明，菌株SQ9-PEA与……密切相关，菌株SQ8-PEA与……和……密切相关，菌株GRT3与……、……和……密切相关。将这些新菌株与……属和……属内系统发育相关物种的模式菌株基因组计算的平均核苷酸同一性和成对数字DNA-DNA杂交值清楚地表明，菌株SQ9-PEA代表……属的一个新物种，菌株SQ8-PEA和GRT3代表……属的两个新物种。这些新型模式菌株的表型特征与其他系统发育相关物种的模式菌株特征不同。基质辅助激光解吸电离飞行时间质谱法支持对这些新物种的鉴定。基于这些数据，我们提出……属的一个新物种，命名为…… sp. nov.，其模式菌株为SQ9-PEA（=DSM 114656=CCUG 76426=NCTC 14727），以及……属的两个新物种，命名为…… sp. nov.，其模式菌株为SQ8-PEA（=DSM 114685=CCUG 76423=NCTC 14723）和…… sp. nov.，其模式菌株为GRT3（=DSM 114696=CCUG 76427=NCTC 14722）。菌株SQ9-PEA、SQ8-PEA和GRT3的基因组G+C含量分别为38.29、36.49和37.26 mol%，完整草图基因组大小分别为1692266、2371088和2237001 bp。
