Everson R B, Randerath E, Santella R M, Avitts T A, Weinstein I B, Randerath K
Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC.
J Natl Cancer Inst. 1988 Jun 15;80(8):567-76. doi: 10.1093/jnci/80.8.567.
Specimens of human placental DNA were tested for chemical addition products (adducts) by recently developed 32P-postlabeling and immunologic assays, and results were compared with data concerning maternal exposures and birth weight. A total of 7 different adducts were detected in the 53 specimens of human placental tissue examined by the 32P-postlabeling assay. Three of these adducts were found almost exclusively in smokers. Among smokers there were positive dose-response relationships between levels of the smoking-related adducts and biochemical estimates of doses of maternal exposure to cigarette smoke during pregnancy. Levels of 1 adduct found only in smokers appeared to relate directly to amounts of caffeine consumption by the mother. In addition to these relationships with maternal exposures, levels of smoking-related adducts were inversely associated with the birth weight of offspring. Results from this study suggest that even at their current formative stage of development, assays for DNA adducts may help identify determinants of DNA damage to human tissues and improve our ability to demonstrate dose-response relationships for the effects of environmental exposures to potentially carcinogenic agents.
采用最近开发的32P后标记法和免疫分析法对人胎盘DNA样本进行化学加成产物（加合物）检测，并将结果与母亲暴露情况和出生体重的数据进行比较。在通过32P后标记法检测的53份人胎盘组织样本中，共检测到7种不同的加合物。其中3种加合物几乎只在吸烟者中发现。在吸烟者中，与吸烟相关的加合物水平与孕期母亲接触香烟烟雾剂量的生化估计值之间存在正剂量反应关系。仅在吸烟者中发现的1种加合物水平似乎与母亲的咖啡因摄入量直接相关。除了这些与母亲暴露的关系外，与吸烟相关的加合物水平与后代出生体重呈负相关。这项研究的结果表明，即使在当前的发展形成阶段，DNA加合物检测也可能有助于识别对人体组织DNA损伤的决定因素，并提高我们证明环境暴露于潜在致癌剂影响的剂量反应关系的能力。
