School of Water and Environment, Chang'an University, Xi'an 710054, China; Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education, Chang'an University, Xi'an 710054, China.
School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Bioresour Technol. 2021 Jun;330:124945. doi: 10.1016/j.biortech.2021.124945. Epub 2021 Mar 10.
The influence of copper ion (Cu) on anaerobic ammonium oxidation (anammox) performance and microbial community structures after oxytetracycline (OTC) stress recovery were assessed. Experimental results demonstrated that anammox performance were stressed by 1.0 mg L Cu and inhibitions were reversible with total nitrogen removal rate higher than 3.08 ± 0.2 kg N m d. The residual OTC in the anammox sludge could combine with Cu introduced and thereby retarded inhibition on performance in the presence of 2.0 mg L Cu. Moreover, the positive relation of dominant bacterium Ca. Anammoxoglobus with the abundance of functional genes and parts of antibiotic resistance genes were observed, suggesting that regain of performance was the results of the gradual domestication of latent resistant species after inhibition. This investigation reveals new insights into resistance of anammox performance for Cu and OTC.
考察了铜离子 (Cu) 对土霉素 (OTC) 胁迫恢复后厌氧氨氧化 (anammox) 性能和微生物群落结构的影响。实验结果表明，1.0mg/L 的 Cu 会对 anammox 性能造成胁迫，抑制作用是可逆的，总氮去除率高于 3.08±0.2kgNmd。在 2.0mg/L Cu 存在的情况下，剩余的 anammox 污泥中的 OTC 可与引入的 Cu 结合，从而减缓对性能的抑制。此外，还观察到优势菌 Ca。Anammoxoglobus 与功能基因和部分抗生素抗性基因的丰度呈正相关，表明在抑制作用后，性能的恢复是潜在抗性物种逐渐驯化的结果。这项研究揭示了 anammox 对 Cu 和 OTC 性能的抗性的新见解。
