Laboratory of Water Pollution Remediation, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Laboratory of Water Pollution Remediation, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Bioresour Technol. 2021 Aug;333:125186. doi: 10.1016/j.biortech.2021.125186. Epub 2021 Apr 16.
Two types of anaerobic ammonium oxidation (anammox) seed sludge were selected to evaluate their responses to copper nanoparticles (CuNPs) exposure. Antibiotic-exposed anammox granules (R) were more likely to be inhibited by 5.0 mg L CuNPs than the normal anammox granules (C). The nitrogen removal efficiency (NRE) of C decreased by 9.00% after two weeks of exposure to CuNPs, whereas that of R decreased by 20.32%. Simultaneously, the abundance of Candidatus. Kuenenia decreased by 27.65% and 36.02% in C and R under CuNPs stress conditions, respectively. Generally, R was more susceptible to CuNPs than C. The correlation analysis indicated that the horizontal transfer of antibiotic resistance genes and copA triggered by intI1 facilitated the generation of multiresistance in the anammox process. Moreover, the potential multiresistance mechanism of anammox bacteria was hypothesized based on previous results. The results will generate new ideas for the treatment of complex wastewater using the anammox process.
两种类型的厌氧氨氧化（anammox）种泥被选择来评估它们对铜纳米颗粒（CuNPs）暴露的反应。暴露于抗生素的厌氧氨氧化颗粒（R）比正常厌氧氨氧化颗粒（C）更容易受到 5.0mg/L CuNPs 的抑制。C 在暴露于 CuNPs 两周后，氮去除效率（NRE）下降了 9.00%，而 R 则下降了 20.32%。同时，在 CuNPs 胁迫条件下，C 和 R 中的 Candidatus. Kuenenia 的丰度分别下降了 27.65%和 36.02%。一般来说，R 比 C 更容易受到 CuNPs 的影响。相关性分析表明，水平转移的抗生素抗性基因和 intI1 触发的 copA 促进了厌氧氨氧化过程中的多抗性产生。此外，根据先前的结果，假设了厌氧氨氧化细菌的潜在多抗性机制。这些结果将为利用厌氧氨氧化工艺处理复杂废水提供新的思路。
