Algae Biotechnology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.
Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam.
Sci Total Environ. 2021 May 10;768:145471. doi: 10.1016/j.scitotenv.2021.145471. Epub 2021 Jan 30.
In this research investigation, three microalgal species were screened (Pleurosigma sp., Amphora sp., and Amphiprora sp.) for lipid content before choosing the potential microalgae for biodiesel production. It was found that the lipid content of Amphiprora sp. was 41.48 ± 0.18%, which was higher than the Pleurosigma sp. (27.3 ± 0.8%) and Amphora sp. (22.49 ± 0.21%). The diatom microalga, Amphiprora sp. was isolated and exposed to a controlled environment. Two different media were prepared, and the main research was on the SiO-NP medium as the cell wall of diatom was made up of silica. Essential growth parameters were studied such as dry cell weight and chlorophyll a content. The results revealed that Amphiprora sp. cultured in the modified medium showed a higher biomass yield and growth rate in all the analyses. In Soxhlet extraction method, biodiesel yield of Amphiprora sp. in modified medium under 24 μmol m s of light intensity was 81.47 ± 1.59% when using 2% of catalyst amount with 1.5:1 volume ratio of methanol/oil in 3 h reaction time at 65 °C. Results reveled that Amphiprora sp. diatom has a higher yield of oil 52.94 ± 0.42% and can be efficiently optimized with further studies with modified nanomaterial culture medium. The present research revealed the series of experiments on microalgal lipid transesterification and in future investigation different types of nanomaterials should be used in culture medium to identify the lipid production in microalgal cells.
在本研究调查中，筛选了三种微藻（Pleurosigma sp.、Amphora sp. 和 Amphiprora sp.）以确定用于生物柴油生产的潜在微藻，然后测定它们的脂质含量。结果发现，Amphiprora sp. 的脂质含量为 41.48±0.18%，高于 Pleurosigma sp.（27.3±0.8%）和 Amphora sp.（22.49±0.21%）。分离出硅藻微藻 Amphiprora sp. 并将其置于受控环境中。制备了两种不同的培养基，并主要研究了 SiO-NP 培养基，因为硅藻的细胞壁由二氧化硅组成。研究了必需的生长参数，如干细胞重量和叶绿素 a 含量。结果表明，在改良培养基中培养的 Amphiprora sp. 在所有分析中均表现出更高的生物量产量和生长速度。在索氏提取法中，在 24 μmol m s 的光强下，使用 2%的催化剂用量和 1.5:1 的甲醇/油体积比，在 65°C 下反应 3 小时，Amphiprora sp. 的生物柴油产率为 81.47±1.59%。结果表明，硅藻 Amphiprora sp. 的产油量更高，为 52.94±0.42%，并且可以通过进一步的研究用改良的纳米材料培养基进行有效优化。本研究对微藻脂质的酯交换进行了一系列实验，在未来的研究中，应该在培养基中使用不同类型的纳米材料来鉴定微藻细胞中的脂质产量。
