Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201301, India.
Algae Biomass and Energy System R&D Center (ABES), Tennodai, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Bioresour Technol. 2021 Jul;332:125098. doi: 10.1016/j.biortech.2021.125098. Epub 2021 Apr 6.
In this work, two benthic marine diatoms Chaetoceros sp. and Thalassiosira sp. were grown in modified f/2 medium in which normal silica was replaced with inductively coupled plasma (ICP) nanosilica for indoor mass cultivation and its impact on growth, lipid content, lipid quality and metabolite production were monitored. Results indicate thatunder mass cultivation using ICP nano silica medium, Thalassiosirasp. reached 3.6 and Chaetoceros sp. reached 3.2-fold higher cell density compared to normal Si medium. The primary metabolite production and total lipid content was higher in Chaetoceros sp. (44.33 ± 2.51% DCW) compared to Thalassiosira sp. (29.66 ± 1.52% DCW). In mass cultivation, ICP synthesized nanosilica powder was effective in enhancing the cell density, production of metabolites, pigments, and lipids in the marine diatoms studied. This is the first report on the use of ICP nanosilica in carrying out indoor mass cultivation of marine diatom isolates as potential biodiesel and biomolecule feedstocks.
在这项工作中，两种底栖海洋硅藻 Chaetoceros sp. 和 Thalassiosira sp. 在改良的 f/2 培养基中生长，该培养基用电感耦合等离子体 (ICP) 纳米硅代替了正常的硅，以进行室内大规模培养，并监测其对生长、脂质含量、脂质质量和代谢产物产生的影响。结果表明，在使用 ICP 纳米硅培养基进行大规模培养时，与正常 Si 培养基相比，Thalassiosira sp. 的细胞密度增加了 3.6 倍，Chaetoceros sp. 的细胞密度增加了 3.2 倍。与 Thalassiosira sp. （29.66±1.52% DCW）相比，Chaetoceros sp. （44.33±2.51% DCW）的初级代谢产物产量和总脂质含量更高。在大规模培养中，ICP 合成的纳米硅粉可有效提高所研究海洋硅藻的细胞密度、代谢产物、色素和脂质的产量。这是首次报道使用 ICP 纳米硅进行海洋硅藻分离物的室内大规模培养，作为潜在的生物柴油和生物分子饲料。
