Indoor mass cultivation of marine diatoms for biodiesel production using induction plasma synthesized nanosilica.

In this work, two benthic marine diatoms Chaetoceros sp. and Thalassiosira sp. were grown in modified f/2 medium in which normal silica was replaced with inductively coupled plasma (ICP) nanosilica for indoor mass cultivation and its impact on growth, lipid content, lipid quality and metabolite production were monitored. Results indicate thatunder mass cultivation using ICP nano silica medium, Thalassiosirasp. reached 3.6 and Chaetoceros sp. reached 3.2-fold higher cell density compared to normal Si medium. The primary metabolite production and total lipid content was higher in Chaetoceros sp. (44.33 ± 2.51% DCW) compared to Thalassiosira sp. (29.66 ± 1.52% DCW). In mass cultivation, ICP synthesized nanosilica powder was effective in enhancing the cell density, production of metabolites, pigments, and lipids in the marine diatoms studied. This is the first report on the use of ICP nanosilica in carrying out indoor mass cultivation of marine diatom isolates as potential biodiesel and biomolecule feedstocks.