有氧锻炼联合氨基葡萄糖治疗和 circUNK 改善兔膝骨关节炎的机制。
The mechanism of aerobic exercise combined with glucosamine therapy and circUNK in improving knee osteoarthritis in rabbits.
机构信息
Department of Joint and Sports Injuries, Guangzhou Tianhe District Chinese Medicine Hospital, China.
Department of Orthopaedics, Yangjiang Hospital of Traditional Chinese Medicine, China.
出版信息
Life Sci. 2021 Jun 15;275:119375. doi: 10.1016/j.lfs.2021.119375. Epub 2021 Mar 15.
AIMS
Both aerobic exercise and glucosamine hydrochloride capsules (OTL) have a therapeutic effect on knee osteoarthritis, but their joint application has not been investigated. This study clarified the mechanism of the combined treatment in knee osteoarthritis.
MAIN METHODS
Aerobic exercise and OTL were used alone or in combination to treat papain-induced knee osteoarthritis model rabbits. Pathological changes of cartilage tissues, inflammatory cytokine content, glycosaminoglycan, and expressions of collagen II, cartilage differentiation-related genes and circUNK were analyzed by hematoxylin-eosin staining, Mankin score, Enzyme-linked immunosorbent assay, toluidine blue staining, Immunohistochemistry and qRT-PCR. The extracted chondrocytes were identified by Alcian Blue staining and immunohistochemistry and induced by iodoacetic acid (MIA) to establish osteoarthritis model. Effects of overexpressing or silencing circUNK on cell function and molecular changes in chondrocytes were analyzed by cell function experiments, qRT-PCR and Western blot. Rabbit modeling and intervention treatment were marked.
KEY FINDINGS
Aerobic exercise or OTL treatment alone relieved the damage caused by knee osteoarthritis in terms of cartilage tissue lesions, Mankin score, inflammatory cytokine content, glycosaminoglycan, and expressions of collagen II, cartilage differentiation-related genes and circUNK. Combined application of aerobic exercise and OTL showed better synergistic treatment effects. Transfection of overexpressed circUNK could attenuate the MIA-induced effect on cell viability and apoptosis in chondrocytes by regulating genes related to differentiation and apoptosis. Aerobic exercise combined with glucosamine had a synergistic therapeutic effect on knee osteoarthritis.
SIGNIFICANCE
Overexpressing circUNK protected osteoarthritis model cells by regulating cartilage differentiation- and apoptosis-related genes.
目的
有氧运动和盐酸氨基葡萄糖胶囊（OTL）对膝骨关节炎均具有治疗作用，但两者联合应用尚未得到研究。本研究阐明了联合治疗膝骨关节炎的机制。
方法
单独或联合使用有氧运动和 OTL 治疗木瓜蛋白酶诱导的膝骨关节炎模型兔。通过苏木精-伊红染色、Mankin 评分、酶联免疫吸附试验、甲苯胺蓝染色、免疫组织化学和 qRT-PCR 分析软骨组织的病理变化、炎性细胞因子含量、糖胺聚糖以及胶原 II、软骨分化相关基因和环状非编码 RNA（circRNA）unk 表达。通过阿尔辛蓝染色和免疫组织化学鉴定提取的软骨细胞，并通过碘乙酸（MIA）诱导建立骨关节炎模型。通过细胞功能实验、qRT-PCR 和 Western blot 分析过表达或沉默 circUNK 对软骨细胞功能和分子变化的影响。对兔建模和干预治疗进行标记。
主要发现
单独的有氧运动或 OTL 治疗可减轻软骨组织损伤、Mankin 评分、炎性细胞因子含量、糖胺聚糖以及胶原 II、软骨分化相关基因和 circRNAunk 表达，从而缓解膝骨关节炎引起的损伤。有氧运动和 OTL 的联合应用显示出更好的协同治疗效果。转染过表达的 circRNAunk 可以通过调节分化和凋亡相关基因来减轻 MIA 对软骨细胞活力和凋亡的诱导作用。有氧运动联合氨基葡萄糖对膝骨关节炎具有协同治疗作用。
意义
过表达的 circRNAunk 通过调节软骨分化和凋亡相关基因来保护骨关节炎模型细胞。
Zotero 插件