National Center of Pathology, Affiliate of Vilnius University Hospital Santaros Clinics, Vilnius, Lithuania.
Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
Sci Rep. 2021 Mar 18;11(1):6275. doi: 10.1038/s41598-021-85667-8.
Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested. We applied polychromatic polarization microscopy to image their spatial organization. Our study provides evidence that IBI are caused by liquid paraffin-macromolecular crystals formed during paraffin-embedding procedures within cells and potentially reflect an active transcriptional status.
核内双折射包涵体（IBI）在石蜡包埋组织切片的各种细胞类型中被发现，长期以来一直被认为是一种组织处理伪影，尽管有人提出了与生物过程的关联。我们应用多色偏振显微镜来成像它们的空间组织。我们的研究提供了证据，证明 IBI 是由细胞内石蜡包埋过程中形成的液态石蜡-大分子晶体引起的，并且可能反映了活跃的转录状态。
