State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, 071000, Hebei, China.
BMC Plant Biol. 2021 Mar 19;21(1):144. doi: 10.1186/s12870-021-02925-7.
Identifying the loci and dissecting the genetic architecture underlying wheat yield- and quality-related traits are essential for wheat breeding. A genome-wide association study was conducted using a high-density 90 K SNP array to analyze the yield- and quality-related traits of 543 bread wheat varieties.
A total of 11,140 polymorphic SNPs were distributed on 21 chromosomes, including 270 significant SNPs associated with 25 yield- and quality-related traits. Additionally, 638 putative candidate genes were detected near the significant SNPs based on BLUP data, including three (TraesCS7A01G482000, TraesCS4B01G343700, and TraesCS6B01G295400) related to spikelet number per spike, diameter of the first internode, and grain volume. The three candidate genes were further analyzed using stage- and tissue- specific gene expression data derived from an RNA-seq analysis. These genes are promising candidates for enhancing yield- and quality-related traits in wheat.
The results of this study provide a new insight to understand the genetic basis of wheat yield and quality. Furthermore, the markers detected in this study may be applicable for marker-assisted selection in wheat breeding programs.
鉴定小麦产量和品质相关性状的基因座并剖析其遗传结构，对于小麦育种至关重要。本研究利用高密度 90K SNP 芯片，对 543 个面包小麦品种的产量和品质相关性状进行了全基因组关联分析。
共检测到 11140 个多态性 SNP，分布在 21 条染色体上，包括 270 个与 25 个产量和品质相关性状显著相关的 SNP。此外，基于 BLUP 数据，在显著 SNP 附近检测到 638 个潜在的候选基因，包括三个与小穗数、第一节间直径和粒体积相关的基因（TraesCS7A01G482000、TraesCS4B01G343700 和 TraesCS6B01G295400）。进一步利用 RNA-seq 分析得到的阶段和组织特异性基因表达数据对这三个候选基因进行了分析。这些基因是提高小麦产量和品质相关性状的有希望的候选基因。
本研究结果为深入了解小麦产量和品质的遗传基础提供了新的认识。此外，本研究中检测到的标记可能适用于小麦育种计划中的标记辅助选择。
