The Federal Research Center Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia.
Kurchatov Genomics Center IC&G SB RAS, Novosibirsk 630090, Russia.
Int J Mol Sci. 2023 Aug 27;24(17):13304. doi: 10.3390/ijms241713304.
The content and quality of gluten in wheat grain is a distinctive characteristic that determines the final properties of wheat flour. In this study, a genome-wide association study (GWAS) was performed on a wheat panel consisting of bread wheat varieties and the introgression lines (ILs) obtained via hybridization with tetraploid wheat relatives. A total of 17 stable quantitative trait nucleotides (QTNs) located on chromosomes 1D, 2A, 2B, 3D, 5A, 6A, 7B, and 7D that explained up to 21% of the phenotypic variation were identified. Among them, the QTLs on chromosomes 2A and 7B were found to contain three and six linked SNP markers, respectively. Comparative analysis of wheat genotypes according to the composition of haplotypes for the three closely linked SNPs of chromosome 2A indicated that haplotype TT/AA/GG was characteristic of ten ILs containing introgressions from . The gluten content in the plants with TT/AA/GG haplotype was significantly higher than in the varieties with haplotype GG/GG/AA. Having compared the newly obtained data with the previously reported quantitative trait loci (QTLs) we inferred that the locus on chromosome 2A inherited from is potentially novel. The introgression lines containing the new locus can be used as sources of genetic factors to improve the quality traits of bread wheat.
小麦籽粒中的面筋含量和质量是决定面粉最终特性的独特特征。本研究利用包含普通小麦品种和通过与四倍体小麦亲缘杂交获得的导入系（ILs）的小麦群体进行了全基因组关联研究（GWAS）。共鉴定出 17 个位于染色体 1D、2A、2B、3D、5A、6A、7B 和 7D 上的稳定数量性状核苷酸（QTNs），它们可以解释高达 21%的表型变异。其中，染色体 2A 和 7B 上的 QTL 分别含有三个和六个连锁 SNP 标记。根据染色体 2A 的三个紧密连锁 SNP 的基因型进行小麦基因型的比较分析表明，TT/AA/GG 单倍型存在于包含来自. 的导入的十个 ILs 中。具有 TT/AA/GG 单倍型的植物的面筋含量明显高于具有 GG/GG/AA 单倍型的品种。将新获得的数据与先前报道的数量性状位点（QTLs）进行比较，我们推断来自. 的染色体 2A 上的位点可能是新的。含有新位点的导入系可作为遗传因子的来源，用于改良普通小麦的品质特性。
