2018 年从伊拉克家养鹅中分离出的高致病性禽流感 A（H5N8）病毒的遗传特征。

Genetic characterization of highly pathogenic avian influenza A (H5N8) virus isolated from domestic geese in Iraq, 2018.

机构信息

College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq.

Molecular Diagnostic Laboratory, Directorate of Veterinary Services, Sulaymaniyah, Kurdistan Region, Iraq.

出版信息

BMC Vet Res. 2021 Mar 19;17(1):124. doi: 10.1186/s12917-021-02831-y.

DOI:10.1186/s12917-021-02831-y

PMID:33740981

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7980536/

Abstract

BACKGROUND

Influenza viruses are a continuous threat to avian and mammalian species, causing epidemics and pandemics. After the circulation of H5N1 in 2006, 2015, and 2016 in Iraq, an H5N8 influenza virus emerged in domestic geese in Sulaymaniyah Province, Iraq. This study analyzed the genetic characteristics of the Iraqi H5N8 viruses.

RESULTS

An HPAI virus subtype H5N8 was identified from domestic backyard geese in the Kurdistan Region, north Iraq. Phylogenic analyses of the hemagglutinin (HA) and neuraminidase (NA) genes indicated that Iraq H5N8 viruses belonged to clade 2.3.4.4 group B and clustered with isolates from Iran, Israel, and Belgium. Genetic analysis of the HA gene indicated molecular markers for avian-type receptors. Characterization of the NA gene showed that the virus had sensitive molecular markers for antiviral drugs.

CONCLUSIONS

This is the first study ever on H5N8 in Iraq, and it is crucial to understand the epidemiology of the viruses in Iraq and the Middle East. The results suggest a possible role of migratory birds in the introduction of HPAI subtype H5N8 into Iraq.

摘要

背景

流感病毒不断威胁着禽类和哺乳动物物种，引发了流感大流行。继 2006 年、2015 年和 2016 年伊拉克出现 H5N1 之后，一种 H5N8 流感病毒出现在伊拉克苏莱曼尼亚省的家养鹅中。本研究分析了伊拉克 H5N8 病毒的遗传特征。

结果

从伊拉克库尔德地区的后院鹅中鉴定出了一种高致病性禽流感病毒亚型 H5N8。血凝素（HA）和神经氨酸酶（NA）基因的系统进化分析表明，伊拉克 H5N8 病毒属于 2.3.4.4 分支 B 群，与来自伊朗、以色列和比利时的分离株聚类。HA 基因的遗传分析表明了对禽源受体的分子标记。NA 基因的特征表明该病毒对抗病毒药物具有敏感的分子标记。