Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Ministry of Agriculture Key Laboratory of Seaweed Fertilizers, Qingdao 266000, China.
Int J Biol Macromol. 2021 Jun 1;180:547-558. doi: 10.1016/j.ijbiomac.2021.03.039. Epub 2021 Mar 16.
A fuciodan (Mw = 11.1 kDa) was obtained and purified from Macrocystis pyrifera (MPF). MPF was an acid heteropolysaccharide including fucose, mannose, xylose, galactose, rhamnose, glucuronic acid, and glucose in a molar ratio of 3.1:1.0:0.86:0.63:0.25:0.33:0.11. Sulfate content in MPF was 28.6%, and the molar ratio of fucose to sulfate (Fuc:SO) was 1.0:0.58. The structure of MPF was mainly consist of repeating →3)-β-L-Fucp (2SO)-(1→ and →4)-β-D-Xylp-(1→3)-β-L-Fucp(2SO)-(1→ and with α-L-Fucp-(1→ and →6)-α-D-Galp-(1→ in branches. Moreover, the effects of different MPF concentrations on plant salt tolerance were investigated. The results indicated that MPF could improve the salt tolerance of wheat seedlings. Among the five concentrations (0.05, 0.1, 0.5, 1, and 2 mg/ml), 0.5 and 1 mg/ml MPF were optimal for effective plant salt-resistance activity. These results suggested that MPF extracted from brown seaweed show potential as plant stimulators that may be used to improve salt resistance of plants.
从巨藻（MPF）中获得并纯化了一种褐藻糖胶（Mw = 11.1 kDa）。MPF 是一种酸性杂多糖，由岩藻糖、甘露糖、木糖、半乳糖、鼠李糖、葡萄糖醛酸和葡萄糖以摩尔比 3.1:1.0:0.86:0.63:0.25:0.33:0.11 组成。MPF 中的硫酸根含量为 28.6%，岩藻糖与硫酸根的摩尔比（Fuc:SO）为 1.0:0.58。MPF 的结构主要由重复的 →3)-β-L-Fucp（2SO）-（1→和 →4）-β-D-Xylp-（1→3）-β-L-Fucp（2SO）-（1→和带分支的 α-L-Fucp-（1→和 →6）-α-D-Galp-（1→组成。此外，还研究了不同 MPF 浓度对植物耐盐性的影响。结果表明，MPF 可以提高小麦幼苗的耐盐性。在五个浓度（0.05、0.1、0.5、1 和 2 mg/ml）中，0.5 和 1 mg/ml 的 MPF 对有效提高植物抗盐性最有效。这些结果表明，从褐藻中提取的褐藻糖胶作为植物刺激物具有潜在应用价值，可用于提高植物的耐盐性。
