Oxidation of various substrates by host grown Mycobacteria leprae and M. lepraemurium.

Oxidation of various substrates by whole cell suspensions of M. Lepraemurium and M. leprae was investigated using manometric techniques. Yeast extract, L-cysteine, dithioerythritol, and DL-penicillamine were oxidized by both M. lepraemurium as well as by M. leprae. Although tween 80 was oxidized by M. lepraemurium cell suspensions, it was not by M. leprae. Succinate was readily oxidized by whole cells of M. leprae (without being frozen) whereas it was oxidized only by M. lepraemurium cells frozen at -40 degrees C for one minute. The results indicate that M. leprae and M. lepraemurium are capable of oxidizing some substrates without requiring any cofactor and are not dependent upon host cells for respiration.