宿主生长的麻风分枝杆菌和鼠麻风分枝杆菌对各种底物的氧化作用。

Oxidation of various substrates by host grown Mycobacteria leprae and M. lepraemurium.

作者信息

Ishaque M, Kato L

出版信息

Rev Can Biol. 1977 Sep;36(3):277-82.

Abstract

Oxidation of various substrates by whole cell suspensions of M. Lepraemurium and M. leprae was investigated using manometric techniques. Yeast extract, L-cysteine, dithioerythritol, and DL-penicillamine were oxidized by both M. lepraemurium as well as by M. leprae. Although tween 80 was oxidized by M. lepraemurium cell suspensions, it was not by M. leprae. Succinate was readily oxidized by whole cells of M. leprae (without being frozen) whereas it was oxidized only by M. lepraemurium cells frozen at -40 degrees C for one minute. The results indicate that M. leprae and M. lepraemurium are capable of oxidizing some substrates without requiring any cofactor and are not dependent upon host cells for respiration.

摘要

利用测压技术研究了鼠麻风分枝杆菌和麻风分枝杆菌全细胞悬液对各种底物的氧化作用。酵母提取物、L-半胱氨酸、二硫赤藓糖醇和DL-青霉胺可被鼠麻风分枝杆菌和麻风分枝杆菌氧化。虽然吐温80可被鼠麻风分枝杆菌细胞悬液氧化，但不能被麻风分枝杆菌氧化。琥珀酸盐很容易被麻风分枝杆菌的全细胞（未冷冻）氧化，而只有在-40℃冷冻一分钟的鼠麻风分枝杆菌细胞才能氧化它。结果表明，麻风分枝杆菌和鼠麻风分枝杆菌能够在不需要任何辅因子的情况下氧化一些底物，并且呼吸不依赖于宿主细胞。