Biology Department, Faculty of Science and Arts in Dahran Aljnoub, King Khalid University, Abha, Saudi Arabia.
Agriculture Research Centre, Soil, Water and Environment Research Institute, Giza, Egypt.
Bull Environ Contam Toxicol. 2021 Jun;106(6):970-977. doi: 10.1007/s00128-021-03190-y. Epub 2021 Mar 20.
The hepatotoxic impacts of 2, 4, and 8 mg/L of AlO nanoparticles (31.4 ± 4.8 nm) were evaluated in Oreochromis niloticus after 7 days of exposure and 15 days of recovery periods. The biochemical analysis of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in plasma showed significant increases in both 4 and 8 mg/L AlO NPs exposed groups. The antioxidant biomarkers showed concentration-dependent elevations in catalase, superoxide dismutase, glutathione peroxidase activities, and thiobarbituric acid reactive substances levels. Glutathione reduced contents showed significant reductions in both 4 and 8 mg/L AlO nanoparticles exposed groups. Several hepatic histopathological alterations were recorded ranging from adaptive responses (e.g. melanomacrophages aggregation) to permanent damage (e.g. necrosis). The recovery period using toxicant-free water led to an obvious reduction in the Al content in liver, liver and antioxidant enzymes in addition to regressive histopathological alterations based on the frequency of alterations occurrence and the extent of affected areas.
在暴露 7 天和恢复 15 天后，评估了 2、4 和 8mg/L 的 AlO 纳米颗粒（31.4±4.8nm）对奥利亚罗非鱼的肝毒性影响。血浆中天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶的生化分析显示，在 4 和 8mg/L AlO NPs 暴露组中均显著增加。抗氧化生物标志物显示，过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性和硫代巴比妥酸反应物质水平呈浓度依赖性升高。还原型谷胱甘肽含量在 4 和 8mg/L AlO 纳米颗粒暴露组中均显著降低。记录了几种肝组织病理学改变，范围从适应性反应（如黑色素巨噬细胞聚集）到永久性损伤（如坏死）。在使用无毒性水的恢复期内，肝脏、肝脏和抗氧化酶中的 Al 含量明显减少，此外，根据改变发生的频率和受影响区域的程度，还出现了退行性组织病理学改变。
