Faculty of Health Science, Department of Nutrition and Dietetics, Sivas Cumhuriyet University, 58140, Sivas, Turkey.
Faculty of Sciences, Department of Biology, Eskisehir Teknik University, 26470, Eskisehir, Turkey.
Arch Microbiol. 2021 Aug;203(6):2831-2841. doi: 10.1007/s00203-021-02273-y. Epub 2021 Mar 20.
This study is aimed at evaluating the probiotic potential of three Enterococcus faecium strains (called 29-P2, 168-P6 and 277-S3) isolated from 'pastırma', a Turkish traditional dry-cured meat product. For this, key probiotic properties and some functional characteristics of strains were tested in vitro. Antimicrobial activity of 3 E. faecium strains was evaluated against 18 indicator microorganisms consisting of 13 foodborne pathogens and 5 lactic acid bacteria and all strains were found as the producer of antimicrobial substance. Especially one strain 168-P6 showed a remarkable activity spectrum and inhibited all of the used foodborne pathogen indicators. Antimicrobial compounds produced by strains were identified by determining the effect of enzyme, pH and temperature on antimicrobial activity. All strains exhibited tolerance to acidic conditions and a simulated gastric environment. Also, strains exhibited high adhesion capacity. The safety of the strains was assessed by determining hemolytic activity and the resistance to 14 different antibiotics. None of the three strains exhibited hemolytic activity, also strains were found reliable in terms of clinically relevant antibiotics, only one strain 29-P2 was found resistant to vancomycin. In addition, metabolic activities of strains including lactic acid, hydrogen peroxide, exopolysaccharide production and proteolytic activity were determined and amounts of all metabolic products were found low. When evaluated all data obtained, it is believed that the strains have enviable characteristics as a probiotic candidate.
本研究旨在评估从土耳其传统干腌肉产品“pastırma”中分离出的 3 株屎肠球菌（称为 29-P2、168-P6 和 277-S3）的益生菌潜力。为此，在体外测试了菌株的关键益生菌特性和一些功能特性。评估了 3 株屎肠球菌对由 13 种食源性致病菌和 5 种乳酸菌组成的 18 种指示微生物的抗菌活性，所有菌株均被鉴定为抗菌物质的产生菌。特别是一株 168-P6 表现出显著的活性谱，抑制了所有使用的食源性致病菌指示菌。通过确定酶、pH 值和温度对抑菌活性的影响，确定了菌株产生的抗菌化合物。所有菌株均表现出对酸性条件和模拟胃液环境的耐受性。此外，菌株还表现出高黏附能力。通过测定溶血活性和对 14 种不同抗生素的耐药性来评估菌株的安全性。这 3 株菌均无溶血活性，而且在临床相关抗生素方面也表现出可靠的特性，只有一株 29-P2 对万古霉素耐药。此外，还测定了菌株的乳酸、过氧化氢、胞外多糖产生和蛋白水解活性等代谢活性，所有代谢产物的含量均较低。综合所有获得的数据，这些菌株具有作为益生菌候选物的令人羡慕的特性。
