评估源自自然发酵香肠的本土屎肠球菌菌株的安全性和益生菌潜力。
Assessment of safety aspect and probiotic potential of autochthonous Enterococcus faecium strains isolated from spontaneous fermented sausage.
机构信息
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000, Kragujevac, Republic of Serbia.
College of Agriculture and Food Technology, Ćirila and Metodija 1, 18400, Prokuplje, Republic of Serbia.
出版信息
Biotechnol Lett. 2020 Aug;42(8):1513-1525. doi: 10.1007/s10529-020-02874-5. Epub 2020 Mar 28.
OBJECTIVE
The objectives of this research project were isolation, identification, and evaluation of the safety aspect and probiotics properties of 21 Enterococcus faecium strains isolated from sausages originated from southeastern Serbia.
RESULTS
Analyzed E. faecium isolates showed tolerance to simulated gastrointestinal conditions. All the examined isolates grew well on media with 0.1% and 0.2% of phenol. None of the tested isolates were histamine-producers, while the synthesis of tyramine was observed for E. faecium sk8-1 and sk8-17. Full resistance to antibiotics was not observed for any examined isolate of E. faecium (penicillin, amoxicillin, and ofloxacin showed the effect on all tested isolates). An inhibition zone against examined pathogens was exhibited by all strains, with the largest inhibition zone against Pseudomonas spp., Proteus spp. and E. coli (12-30 mm/MIC values ranged from 0.5 to 12 mg mL).
CONCLUSION
The results indicated that E. faecium isolates from spontaneously fermented sausage showed a potential for further investigation and possible application as probiotics.
目的
本研究项目的目的是从塞尔维亚东南部香肠中分离、鉴定和评估 21 株屎肠球菌的安全性和益生菌特性。
结果
分析的屎肠球菌分离株对模拟胃肠道条件具有耐受性。所有检测到的分离株在含有 0.1%和 0.2%苯酚的培养基上生长良好。没有测试的分离株是组胺产生菌，而 E. faecium sk8-1 和 sk8-17 则合成了酪胺。没有任何一种屎肠球菌（青霉素、阿莫西林和氧氟沙星对所有测试的分离株都有作用）对所检测到的抗生素完全耐药。所有菌株都对被检测到的病原体表现出抑制作用，对假单胞菌、变形菌和大肠杆菌的抑制圈最大（12-30 毫米/MIC 值范围为 0.5 至 12 毫克/毫升）。
结论
结果表明，来自自然发酵香肠的屎肠球菌具有进一步研究和可能作为益生菌应用的潜力。
Zotero 插件