Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, India.
Institute of Chemical Technology, Marathwada Campus, Jalna, India.
World J Microbiol Biotechnol. 2022 Sep 7;38(12):219. doi: 10.1007/s11274-022-03404-3.
Enterococcus bacteria are studied in various sectors including fermentation, food and dairy industries,as well as studied for their probiotic properties but have limited use due to their possible pathogenic behavior. The present report talks about the metabolites produced, by the previously isolated Enterococcus strain, E.villorum SB2 (accession number KX830968), from the vaginal source. The growth of the bacteria in three types of media (M17, MRS and LAPTg) was compared, where the M17 media gave better bacterial colonies, also maximum growth rate was observed in M17 media (T = 1.6 h & k = 0.4 h), and thus was selected as the metabolite production media. Further, the studied bacteria did not show any hemolytic activity, making it safe for industrial applications. The HR-LCMS results showed the production of various amino acids, organic acids, peptides, and other metabolites like flavonoids (Quercetin 3-O-Manoglucoside), terpenoids (7',8',Dihydro-8'-hydroxycitraniaxanthin, O-Methylganoderic acid O, Thalicsessine, Austinol, Valdiate), indole derivatives produced by tryptophan metabolism (5-hydroxykynurenamine, 2S,4R)-4-(9H-Pyrido[3,4-b]indol-1-yl)-1,2,4-butanetriol, Indoleacrylic acid), antimicrobial compounds (Fortimicin A) and fatty acids (Stearic acid, Myristic acid), which were earlier unreported form Enterococcus species opening new scope for discovering new industrial applications of the strain. As the studied bacteria has been reported to be a potential probiotic, the detection of these industrially important metabolites can be studied further in future studies to reveal the potential industrial applications of the strain.
肠球菌细菌在各个领域都有研究，包括发酵、食品和乳制品行业，也因其益生菌特性而被研究，但由于其可能的致病行为，其应用有限。本报告介绍了先前从阴道来源分离的肠球菌菌株 E.villorum SB2（登录号 KX830968）产生的代谢产物。比较了细菌在三种培养基（M17、MRS 和 LAPTg）中的生长情况，其中 M17 培养基产生的细菌菌落更好，在 M17 培养基中观察到最大生长速率（T=1.6 h 和 k=0.4 h），因此选择其作为代谢产物生产培养基。此外，研究的细菌没有表现出任何溶血活性，使其可安全用于工业应用。HR-LCMS 结果表明，产生了各种氨基酸、有机酸、肽和其他代谢产物，如类黄酮（槲皮素 3-O-甘露糖苷）、三萜类（7'，8'，二氢-8'-羟基柠檬酸嗪，O-甲基 Ganoderic 酸 O，Thalicsessine，Austinol，Valdate）、色氨酸代谢产生的吲哚衍生物（5-羟基犬尿氨酸，（2S，4R）-4-（9H-吡啶并[3,4-b]吲哚-1-基）-1,2,4-丁三醇，吲哚丙烯酸）、抗菌化合物（Fortimicin A）和脂肪酸（硬脂酸，肉豆蔻酸），这些都是肠球菌属以前未报道的形式，为该菌株的新的工业应用开辟了新的前景。由于研究的细菌已被报道为一种潜在的益生菌，因此可以在未来的研究中进一步研究这些具有工业重要性的代谢产物的检测，以揭示该菌株的潜在工业应用。
