Department of Cardiology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shaanxi, China.
Department of Cardiology, Xi'An International Medical Center Hospital, Xi'an, Shaanxi, China.
J Tissue Eng Regen Med. 2021 May;15(5):453-462. doi: 10.1002/term.3184. Epub 2021 Mar 20.
Accumulating evidence has suggested that mechanical stimuli play a crucial role in regulating the lineage-specific differentiation of stem cells through fine-tuning redox balance. We aimed to investigate the effects of cyclic tensile strain (CTS) on the expression of antioxidant enzymes and cardiac-specific genes in P19 cells, a widely characterized tool for cardiac differentiation research. A stretching device was applied to generate different magnitude and duration of cyclic strains on P19 cells. The messenger RNA and protein levels of targeted genes were determined by real-time polymerase chain reaction and Western blot assays, respectively. Proper magnitude and duration of cognitive stimulation therapy (CST) stimulation substantially enhanced the expression of both antioxidant enzymes and cardiac-specific genes in P19 cells. Sirtuin 1 (SIRT1) played an essential role in the CTS-induced cardiomyogenic differentiation of P19, as evidenced by changes in the expression of antioxidant enzymes and cardiac-specific genes. Mechanical loading promoted the cardiomyogenic differentiation of P19 cells. SIRT1 was involved in CST-mediated P19 differentiation, implying that SIRT1 might serve as an important target for developing methods to promote cardiomyogenic differentiation of stem cells.
越来越多的证据表明，机械刺激通过精细调节氧化还原平衡，在调节干细胞的谱系特异性分化中起着至关重要的作用。我们旨在研究循环拉伸应变（CTS）对 P19 细胞中抗氧化酶和心脏特异性基因表达的影响，P19 细胞是一种广泛用于心脏分化研究的工具。拉伸装置用于在 P19 细胞上产生不同幅度和持续时间的循环应变。通过实时聚合酶链反应和 Western blot 分析分别测定靶向基因的信使 RNA 和蛋白质水平。适当幅度和持续时间的认知刺激疗法（CST）刺激可显著增强 P19 细胞中抗氧化酶和心脏特异性基因的表达。Sirtuin 1（SIRT1）在 CTS 诱导的 P19 心肌生成分化中起着至关重要的作用，这可以通过抗氧化酶和心脏特异性基因表达的变化来证明。机械加载促进了 P19 细胞的心肌生成分化。SIRT1 参与了 CST 介导的 P19 分化，这表明 SIRT1 可能是开发促进干细胞心肌生成分化方法的重要靶点。
