Wang Ziqi, Gao Lilan, Lyu Linwei, Wang Xin, Zhang Chunqiu
Tianjin Key Laboratory of Advanced Mechatronic System Design and Intelligent Control, Tianjin University of Technology, Tianjin 300384, P. R. China.
National Experimental Teaching Demonstration Center of Mechatronics Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2022 Oct 25;39(5):997-1004. doi: 10.7507/1001-5515.202204008.
Based on the current study of the influence of mechanical factors on cell behavior which relies heavily on experiments , a culture chamber with a large uniform strain area containing a linear motor-powered, up-to-20-Hz cell stretch loading device was developed to exert mechanical effects on cells. In this paper, using the strain uniformity as the target and the substrate thickness as the variable, the substrate bottom of the conventional incubation chamber is optimized by using finite element technique, and finally a new three-dimensional model of the incubation chamber with "M" type structure in the section is constructed, and the distribution of strain and displacement fields are detected by 3D-DIC to verify the numerical simulation results. The experimental results showed that the new cell culture chamber increased the accuracy and homogeneous area of strain loading by 49.13% to 52.45% compared with that before optimization. In addition, the morphological changes of tongue squamous carcinoma cells under the same strain and different loading times were initially studied using this novel culture chamber. In conclusion, the novel cell culture chamber constructed in this paper combines the advantages of previous techniques to deliver uniform and accurate strains for a wide range of cell mechanobiology studies.
基于目前对机械因素对细胞行为影响的研究（该研究严重依赖实验），开发了一种具有大均匀应变区域的培养室，其包含线性电机驱动、高达20赫兹的细胞拉伸加载装置，以对细胞施加机械作用。本文以应变均匀性为目标、以基底厚度为变量，利用有限元技术对传统培养箱的基底底部进行优化，最终构建了一种截面为“M”型结构的新型培养箱三维模型，并通过3D-DIC检测应变和位移场的分布，以验证数值模拟结果。实验结果表明，新型细胞培养箱与优化前相比，应变加载的精度和均匀区域提高了49.13%至52.45%。此外，使用这种新型培养箱初步研究了相同应变和不同加载时间下舌鳞状癌细胞的形态变化。总之，本文构建的新型细胞培养箱结合了先前技术的优点，可为广泛的细胞力学生物学研究提供均匀且精确的应变。
